Graduate Program in Oral Science, Periodontology Unit, School of Dentistry, Federal University of Santa Maria, Marechal Floriano Peixoto 1184, Santa Maria, Rio Grande do Sul, 97015-372, Brazil.
Graduate Program in Oral Science, Pathology Department, School of Dentistry, Federal University of Santa Maria, Faixa de Camobi, Km 09, Campus Universitário, Santa Maria, Rio Grande do Sul, 97105-900, Brazil.
Lasers Med Sci. 2019 Aug;34(6):1253-1260. doi: 10.1007/s10103-019-02733-4. Epub 2019 Feb 8.
The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment, n = 5), SRPG (SRP, n = 10), aPDTW (SRP+aPDT-MB/water, n = 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose, n = 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (p < 0.05). Percentage of animals that presented mild and severe inflammatory infiltrate was 10% and 40% for SRPG, 20% and 30% for aPDTW, and 50% and 0% for aPDTEt, respectively. BL area (mm) was statistically higher in the LG (0.39 ± 0.15) than NLG (0.05 ± 0.02). aPDTEt showed the lowest value of BL (0.08 ± 0.03), followed by aPDTW (0.21 ± 0.15) and SRPG (0.31 ± 0.18). Statistical differences were verified between aPDTEt and SRPG. In relation to the LG, aPDTEt, aPDTW, and SRPG recovered the equivalent 80%, 46%, and 20% of the BL. aPDTEt showed collagen content statistically higher than SRPG and LG, and presented higher mean values than NLG (p > 0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.
本研究旨在评估 MB 实验制剂（20%乙醇）在辅助牙周治疗中的应用效果，具体为作为牙周基础治疗（SRP）的辅助治疗，辅助治疗对象为糖尿病大鼠。40 只雄性 Wistar 大鼠接受链脲佐菌素腹腔注射以诱导糖尿病。14 天后，5 只动物被分配到非结扎组（NLG），35 只动物在第一右下颌磨牙处结扎以诱导牙周炎。7 天后，结扎被去除，动物被随机分为 4 组：LG（无治疗，n=5）、SRPG（SRP，n=10）、aPDTW（SRP+aPDT-MB/水，n=10）和 aPDTEt（SRP+aPDT-MB/水/乙醇/羧甲基纤维素，n=10）。动物在 7 天后处死。对骨丧失（BL）面积、炎症细胞反应程度和胶原纤维百分比的数据进行了统计学分析（p<0.05）。出现轻度和重度炎症浸润的动物百分比分别为 SRPG 组的 10%和 40%、aPDTW 组的 20%和 30%、aPDTEt 组的 50%和 0%。LG 组（0.39±0.15）的 BL 面积（mm）明显高于 NLG 组（0.05±0.02）。aPDTEt 组的 BL 最小（0.08±0.03），其次是 aPDTW 组（0.21±0.15）和 SRPG 组（0.31±0.18）。aPDTEt 与 SRPG 之间存在统计学差异。与 LG 相比，aPDTEt、aPDTW 和 SRPG 分别恢复了 BL 的 80%、46%和 20%。aPDTEt 组的胶原含量明显高于 SRPG 组和 LG 组，且高于 NLG 组（p>0.05）。我们的研究结果表明 aPDTEt 具有较好的应用前景。含乙醇的 MB-aPDT 可作为糖尿病患者牙周辅助治疗的一种选择。
