Barin L M, Barcelos R C S, Vey L T, Pillusky F M, Palma V M, Kantorski K Z, Bürger M E, Maciel R M, Danesi C C
Post-Graduation Program in Dental Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Post-Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
J Photochem Photobiol B. 2017 Aug;173:538-544. doi: 10.1016/j.jphotobiol.2017.06.039. Epub 2017 Jun 28.
To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model.
Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis.
PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels.
In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment.
首次在体内评估溶解于乙醇的亚甲蓝（MB）光敏剂在抗菌光动力疗法（aPDT）中作为辅助牙周治疗的效果，以及对大鼠模型血浆氧化应激和血管行为的影响。
将Wistar大鼠分为阴性对照组（NC，无牙周炎）和阳性对照组（PC，有牙周炎，未进行任何治疗）。其他组患有牙周炎，并接受了龈下刮治和根面平整（SRP）；SRP+aPDT+溶解于水的MB（aPDT I）；SRP+aPDT+溶解于乙醇的MB（aPDT II）。通过结扎下颌右侧第一磨牙诱导牙周炎。在第7/15/30天，对大鼠实施安乐死，采集血浆用于测定氧化应激参数，采集牙龈组织进行组织形态计量分析。
PC组在第7/15/30天的硫代巴比妥酸反应性物质水平较高。aPDT II能够阻止脂质过氧化，尤其是在第15天至第30天之间。在整个实验过程中，PC组、aPDT I组和II组的谷胱甘肽还原水平均降低。aPDT II组维生素C水平升高，在第30天该组维生素C水平恢复。aPDT II组的血管数量最多。
综上所述，溶解于乙醇的MB进行aPDT在牙周炎治疗中提供了更好的治疗反应。
