小鼠胚胎及组织切片中基因与蛋白质表达的检测

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.

作者信息

McGlinn Edwina, Holzman Miriam A, Mansfield Jennifer H

机构信息

EMBL Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.

Department of Biology, Barnard College, Columbia University, New York, NY, USA.

出版信息

Methods Mol Biol. 2019;1920:183-218. doi: 10.1007/978-1-4939-9009-2_12.

DOI:10.1007/978-1-4939-9009-2_12

PMID:30737693

Abstract

Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.

摘要

基因（mRNA和蛋白质）表达模式的分析是胚胎发育研究的核心。本章详细介绍了在全小鼠胚胎和组织切片中检测mRNA和蛋白质表达的方法，包括mRNA原位杂交、免疫组织化学以及酶和荧光蛋白报告基因的检测。我们重点关注组织学方法；这里不包括测量基因表达的分子方法（例如，RNA测序、PCR）。