McGlinn Edwina, Holzman Miriam A, Mansfield Jennifer H
EMBL Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
Department of Biology, Barnard College, Columbia University, New York, NY, USA.
Methods Mol Biol. 2019;1920:183-218. doi: 10.1007/978-1-4939-9009-2_12.
Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.
基因（mRNA和蛋白质）表达模式的分析是胚胎发育研究的核心。本章详细介绍了在全小鼠胚胎和组织切片中检测mRNA和蛋白质表达的方法，包括mRNA原位杂交、免疫组织化学以及酶和荧光蛋白报告基因的检测。我们重点关注组织学方法；这里不包括测量基因表达的分子方法（例如，RNA测序、PCR）。
