Zahid Maria, Rinas Ursula
Technical Chemistry-Life Science, Leibniz University of Hannover, Hannover, Germany.
Department of Biology, Lahore University of Management Sciences, Lahore, Pakistan.
Methods Mol Biol. 2019;1923:309-322. doi: 10.1007/978-1-4939-9024-5_14.
Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
基于病毒样颗粒（VLP）的疫苗已经在市场上存在了数十年，用于预防病毒感染，并且在生物技术的其他领域也证明了其有效性。在这里，我们详细描述了以毕赤酵母为表达宿主生产和纯化乙型肝炎表面抗原（HBsAg）VLP的简单小规模程序。该方案经过修改后也可能适用于其他基于HBsAg的VLP，这些VLP还携带其他病原体的抗原。
