UNESP, São Paulo State University, Botucatu Medical School, Department of Pathology, Botucatu, São Paulo, Brazil.
UNESP, São Paulo State University, Botucatu Medical School, Department of Pathology, Botucatu, São Paulo, Brazil; Centro Universitário Cesmac, Maceió, Alagoas, Brazil.
Reprod Toxicol. 2019 Apr;85:59-64. doi: 10.1016/j.reprotox.2019.02.001. Epub 2019 Feb 6.
We investigated whether mitochondrial-related genes and proteins are modulated by hyperglycemia promoted by gestational diabetes (GDM), thereby increasing neonate obesity predisposition. 19 healthy pregnant women, 16 pregnant women with GDM and their respective neonates were enrolled. Additionally, 19 obese and 19 eutrophic adults were recruited as a reference population. Umbilical cord, peripheral blood and placental (villous and decidua) tissues were collected to evaluate SOD2, PPAR-α and PPARGC-1β and their respective protein expressions. Data from the reference population confirmed that the three genes and proteins were overexpressed in blood cells of obese compared to eutrophic subjects. Only SOD2 was found upregulated in placental villous (fetal side) tissue of GDM women. Therefore, our findings showed an interaction between the hyperglycemic environment and SOD2 modulation, but also indicated that none of the three genes is useful as potential biomarkers for obesity development.
我们研究了由妊娠期糖尿病（GDM）引起的高血糖是否会调节与线粒体相关的基因和蛋白，从而增加新生儿肥胖易感性。我们招募了 19 名健康孕妇、16 名患有 GDM 的孕妇及其各自的新生儿，此外还招募了 19 名肥胖和 19 名营养良好的成年人作为参考人群。采集脐带、外周血和胎盘（绒毛和蜕膜）组织以评估 SOD2、PPAR-α 和 PPARGC-1β 及其各自的蛋白表达。参考人群的数据证实，与营养良好的受试者相比，肥胖受试者的血细胞中这三种基因和蛋白表达过度。仅发现 SOD2 在 GDM 妇女的胎盘绒毛（胎儿侧）组织中上调。因此，我们的研究结果表明高血糖环境与 SOD2 调节之间存在相互作用，但也表明这三种基因均不能作为肥胖发展的潜在生物标志物。
