Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Photodiagnosis Photodyn Ther. 2019 Mar;25:472-479. doi: 10.1016/j.pdpdt.2019.02.006. Epub 2019 Feb 6.
Some materials such as TiO display a luminescence property when exposed to X-ray radiation. Therefore, a proper photosensitizer can induce photodynamic effects by absorbing the emitted photons from these materials during radiotherapy. In this way, the problem of limited photo- penetration depth in photodynamic therapy is resolved. In this paper, following the production of a nanopolymer containing TiO2 cores and imprinted for mitoxantron (MIP), the possibility of utilizing its optical and radio properties on two lines of cancer cells were studied.
Mitoxantron (MX) was selected as the photosensitizer. The emission spectrum of the nanopolymers synthesized with/without MX was recorded during excitation by 6 MV X-rays. Also, the fluorescence signal of hydroxyl radicals produced into terephthalic acid medium by the nanopolymers were recorded during X irradiation. The percentage of cell survival following irradiation by X-rays was determined for various concentrations of drug agents by MTT assay. The synergistic index and IC were calculated to compare the findings.
The emission spectrum of the nanopolymer reloaded with MX during X-ray irradiation indicated a considerable decline in comparison with the nanopolymer without MX. The level of free radicals produced by nanopolymer was significantly increased during irradiation with X-rays. The highest mean of synergistic indexes was observed in MIP.
The higher level of hydroxyl free radicals in MIP and lower cell viability in the DFW cell line as well as enhanced treatment efficiency confirm the hypothesis regarding the production of photodynamic effects by synthesized nanopolymer during radiotherapy.
某些材料，如 TiO2，在受到 X 射线辐射时会显示出发光性能。因此，适当的光敏剂可以通过在放射治疗期间吸收这些材料发出的光子来诱导光动力效应。这样，就解决了光动力疗法中光穿透深度有限的问题。在本文中，制备了一种含有 TiO2 核并对米托蒽醌（MIP）进行印迹的纳米聚合物后，研究了其在两条癌细胞系上利用其光学和放射学性质的可能性。
选择米托蒽醌（MX）作为光敏剂。记录了合成的纳米聚合物在 6 MV X 射线激发下有无 MX 时的发射光谱。此外，还记录了纳米聚合物在 X 射线照射下产生的对苯二甲酸介质中羟基自由基的荧光信号。通过 MTT 测定法确定不同药物浓度照射 X 射线后细胞存活率的百分比。计算协同指数和 IC 以比较结果。
载有 MX 的纳米聚合物在 X 射线照射下的发射光谱与不含 MX 的纳米聚合物相比明显下降。X 射线照射时，纳米聚合物产生的自由基水平显著增加。MIP 中观察到的协同指数平均值最高。
MIP 中羟基自由基水平较高，DFW 细胞系中细胞活力较低，治疗效率提高，证实了在放射治疗过程中合成纳米聚合物产生光动力效应的假设。
