Sazgarnia Ameneh, Bahreyni-Toosi Mohammad Hossein, Montazerabadi Ali Reza, Ahmadi Amirhossein
Department of Medical Physics, Research Center of Medical Physics, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Physics, Tehran University of Medical Sciences, Tehran, Iran.
J Exp Ther Oncol. 2013;10(3):189-96.
This study aimed to evaluate the effects of indocyanine green as a sensitizer for both photodynamic and radiation therapy in the DFW human melanoma cell line. The cells were incubated with indocyanine green at different concentrations for 24 hours and then exposed in independent treatment groups to non-coherent light at different fluence rates and X-ray ionizing radiation at different dose rates. In addition, the combined effects of this chemo-, photo-, and radiotherapy were evaluated using the MTT assay. The results showed that indocyanine green had no significant cytotoxic effects at concentrations up to 100 microM. However, when the compound was used as a photosensitizer, it had a strong cytotoxic effect on cancer cells. No radiosensitizing activity was detected. Surprisingly, treatment with 50 microM indocyanine green in combination with 30 J/cm2 light and 4 Gy X-ray radiation reduced the percentage of viable cancer cells to 1.22%. The inclusion of the photosensitizer with the low dose of radiation and reduced light fluence rate therefore yielded the same treatment efficacy as more toxic, high-dose radiation and light therapies.
本研究旨在评估吲哚菁绿作为一种敏化剂在DFW人黑色素瘤细胞系中对光动力疗法和放射疗法的效果。将细胞与不同浓度的吲哚菁绿孵育24小时，然后在独立的治疗组中分别以不同的通量率暴露于非相干光下，并以不同的剂量率暴露于X射线电离辐射下。此外，使用MTT法评估这种化学疗法、光动力疗法和放射疗法的联合效果。结果表明，吲哚菁绿在浓度高达100微摩尔时无显著细胞毒性作用。然而，当该化合物用作光敏剂时，它对癌细胞具有强烈的细胞毒性作用。未检测到放射增敏活性。令人惊讶的是，用50微摩尔的吲哚菁绿联合30焦每平方厘米的光和4戈瑞的X射线辐射进行治疗，可将存活癌细胞的百分比降低至1.22%。因此，加入光敏剂并降低辐射剂量和光通量率可产生与毒性更强的高剂量辐射和光疗法相同的治疗效果。
