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3D8单链可变片段蛋白抑制传染性支气管炎病毒在转基因鸡中的传播。

The 3D8 single chain variable fragment protein suppress infectious bronchitis virus transmission in the transgenic chickens.

作者信息

Lee Gunsup, Choi Hoonsung, Sureshkumar Shanmugam, Jung Sun Keun, Kim Jeom Sun, Oh Keon Bong, Kim Kyung-Woon, Yang Hyeon, Kim Dong-Hoon, Byun Sung June

机构信息

Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, 1500, Wanju-gun 441-706, Republic of Korea; Gyeongbuk Institute for Marine Bio-Industry (GIMB), Uljin 36315, Gyeongbuk, Republic of Korea.

Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, 1500, Wanju-gun 441-706, Republic of Korea.

出版信息

Res Vet Sci. 2019 Apr;123:293-297. doi: 10.1016/j.rvsc.2019.01.025. Epub 2019 Jan 23.

DOI:10.1016/j.rvsc.2019.01.025
PMID:30738233
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7111750/
Abstract

Infectious bronchitis (IB) generated by the infectious bronchitis virus (IBV) causes economic difficulties for livestock farmers. The 3D8 single chain variable fragment (scFv) protein is a recombinant antibody with nuclease activity that shows antiviral effects against various DNA and RNA viruses in mice and chickens. In this experiment, 3D8 scFv G transgenic chickens produced by crossing 3D8 scFv G transgenic rooster and wild type hens were screened by genomic PCR and immunohistochemistry analysis. 3D8 scFv transgenic chickens, wild type sibling chickens, and SPF chickens were directly infected with IBV (5 chickens per group) and indirectly infected by airborne propagation (15 chickens per group). The relative IBV shedding titers were measured by quantitative real-time PCR using oropharyngeal and cloacal swabs on days 3 and 5 after intraocular infection. The viral load was significantly decreased in the 3D8 scFv transgenic chickens from the contact transmission group. Additionally, blood was collected from each group on day 17 post-infection. The ELISA results showed a marked reduction of the antibody titer against IBV in the 3D8 scFv transgenic chickens from the contact transmission group. These results suggest that the 3D8 scFv protein potentially inhibits infectious bronchitis virus transmission in chickens.

摘要

传染性支气管炎病毒（IBV）引发的传染性支气管炎（IB）给养殖户带来了经济困难。3D8单链可变片段（scFv）蛋白是一种具有核酸酶活性的重组抗体，在小鼠和鸡体内对多种DNA和RNA病毒均显示出抗病毒作用。在本实验中，通过基因组PCR和免疫组化分析，对由3D8 scFv G转基因公鸡与野生型母鸡杂交产生的3D8 scFv G转基因鸡进行了筛选。将3D8 scFv转基因鸡、野生型同胞鸡和无特定病原体（SPF）鸡直接感染IBV（每组5只鸡），并通过空气传播进行间接感染（每组15只鸡）。在眼内感染后第3天和第5天，使用口咽和泄殖腔拭子通过定量实时PCR测量相对IBV排毒滴度。接触传播组的3D8 scFv转基因鸡的病毒载量显著降低。此外，在感染后第17天从每组采集血液。ELISA结果显示，接触传播组的3D8 scFv转基因鸡中针对IBV的抗体滴度明显降低。这些结果表明，3D8 scFv蛋白可能抑制鸡传染性支气管炎病毒的传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/26d4ea9a44c7/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/62f4f3159dc4/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/1ad037223a60/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/26d4ea9a44c7/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/62f4f3159dc4/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/1ad037223a60/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a0/7111750/26d4ea9a44c7/gr3_lrg.jpg

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Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus.鸡甘露糖结合凝集素对传染性支气管炎病毒具有抗病毒活性。
Virology. 2017 Sep;509:252-259. doi: 10.1016/j.virol.2017.06.028. Epub 2017 Jul 4.
3
The effect of Allium sativum (Garlic) extract on infectious bronchitis virus in specific pathogen free embryonic egg.
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Avicenna J Phytomed. 2016 Jul-Aug;6(4):458-267.
4
Comparison between dot-immunoblotting assay and clinical sign determination method for quantifying avian infectious bronchitis virus vaccine by titration in embryonated eggs.斑点免疫印迹法与临床症状判定法在通过鸡胚滴定定量检测禽传染性支气管炎病毒疫苗中的比较。
J Virol Methods. 2016 Apr;230:13-17. doi: 10.1016/j.jviromet.2016.01.008. Epub 2016 Jan 21.
5
Therapeutic Strategy for the Prevention of Pseudorabies Virus Infection in C57BL/6 Mice by 3D8 scFv with Intrinsic Nuclease Activity.具有内在核酸酶活性的3D8单链抗体片段预防C57BL/6小鼠伪狂犬病病毒感染的治疗策略
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6
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