[Th9、Th17、调节性T细胞水平及IL-9、IL-17和转化生长因子-β表达在特发性血小板减少性紫癜患者外周血中的作用及在特发性血小板减少性紫癜发病机制中的作用]
[Role of Th9, Th17, Treg Cells levels and IL-9, IL-17 and TGF-β Expression in Peripheral Blood of Patients with ITP in Pathogenesis of ITP].
作者信息
Zhou Zheng-Ju, Zhang Yi-Shan, Liang Cai-Xia, Yang Zhang-Yuan
机构信息
Laboratory of Hematologyic Oncotosy.The First People's Hospital of Jingzhou in Hubei Province, Jingzhou 434000, Hubei Province, China.E-mail:
Department of Hematology.The First People's Hospital of Jingzhou in Hubei Province, Jingzhou 434000, Hubei Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Feb;27(1):180-184. doi: 10.7534/j.issn.1009-2137.2019.01.029.
OBJECTIVE
To detect the levels of Treg, Th17, Th9 cells and expression of transforming growth factor-β (TGF-β), interleukin-17 (IL-17) and interleukin-9 (IL-9) in peripheral blood of patients with immune thrombocytopenia (ITP) and to explore its role in the pathogenesis of ITP.
METHODS
Fifty-four patients with ITP (ITP group) and 40 healthy volunteers (control group) were selected in our hospital. The of Treg, Th17 and Th9 cells in peripheral blood of 2 groups were measured by flow cytometry, and the expression of cytokines, such as TGF-β, IL-17 and IL-9 in the peripheral blood of 2 groups were detected by enzyme linked immunosorbent assay (ELISA).
RESULTS
The level of Treg cells in the peripheral blood of the ITP group was significantly decreased in comparison with the control group, while the levels of Th17 and Th9 cells significantly increased in comparison with the control group (all P<0.01). The expression of cytokine such as TGF-β in the peripheral blood of the case group significantly decreased in comparison with the control group, while the expression levels of IL-17 and IL-9 significantly increased in comparison with the control group (P<0.01). The results of Pearson correlation analysis showed that there was a positive correlation between the level of Treg cells and platelet count (PLT) in peripheral blood of the ITP group (r=0.35, P<0.05), and there were negative correlation between the level rate of Th17, Th9 cells and Plt count (r=-0.37, -0.43, P<0.05); there was a positive correlation between the expression of the TGF-β in the ITP group and Plt count (r=0.46, P<0.05), while the expression of IL-17 and IL-9 showed negative correlation with PLT (r=-0.48, -0.54, P<0.05).
CONCLUSION
The percentage of Treg, Th17 and Th9 cells in the peripheral blood of patients with ITP is abnormal, and the expression of TGF-β, IL-17 and IL-9 also is abnormal, which may play an important role in the pathogenesis of ITP.
目的
检测免疫性血小板减少症（ITP）患者外周血中调节性T细胞（Treg）、辅助性T细胞17（Th17）、辅助性T细胞9（Th9）水平及转化生长因子-β（TGF-β）、白细胞介素-17（IL-17）和白细胞介素-9（IL-9）的表达，探讨其在ITP发病机制中的作用。
方法
选取我院54例ITP患者（ITP组）和40例健康志愿者（对照组）。采用流式细胞术检测两组外周血中Treg、Th17和Th9细胞水平，采用酶联免疫吸附测定（ELISA）法检测两组外周血中TGF-β、IL-17和IL-9等细胞因子的表达。
结果
ITP组外周血中Treg细胞水平较对照组显著降低，而Th17和Th9细胞水平较对照组显著升高（均P<0.01）。病例组外周血中TGF-β等细胞因子表达较对照组显著降低，而IL-17和IL-9表达水平较对照组显著升高（P<0.01）。Pearson相关性分析结果显示，ITP组外周血中Treg细胞水平与血小板计数（PLT）呈正相关（r=0.35，P<0.05），Th17、Th9细胞水平与Plt计数呈负相关（r=-0.37，-0.43，P<0.05）；ITP组中TGF-β表达与Plt计数呈正相关（r=0.46，P<0.05），而IL-17和IL-9表达与PLT呈负相关（r=-0.48，-0.54，P<0.05）。
结论
ITP患者外周血中Treg、Th17和Th9细胞百分比异常，TGF-β、IL-17和IL-9表达也异常，这可能在ITP发病机制中起重要作用。
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