人脐带间充质干细胞来源外泌体对调节性T细胞和辅助性T细胞17的调控作用
[Regulatory Effect of Exosomes Derived from Human Umbiilcal Cord Mesenchymal Stem Cells on Treg and TH17 Cells].
作者信息
Guo Li-Yan, Lai Pei-Long, Geng Su-Xia, Chen Xiao-Mei, Wang Yu-Lian, Huang Tian, DU Xin, Weng Jian-Yu
机构信息
School of Medicine, South China University of Technology; Guangzhou 510006, Guangdong Province, China.Department of Hematology, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangdong Elderly Research Institute, Guangzhou 510080, Guangdong Province, China.
Department of Hematology, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangdong Elderly Research Institute, Guangzhou 510080, Guangdong Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Feb;27(1):221-226. doi: 10.7534/j.issn.1009-2137.2019.01.036.
OBJECTIVE
To investigate the effects of exosomes from human umbilical cord mesenchymal stem cells on the development of Treg and TH17 cells.
METHODS
Exosomes from the serum-free-culture supernatants of hUC-MSC were harvested by ultracentrifugation. The electron microscopy, nanoparticle tracking analysis and western blot were used to identify the hUC-MSC-exosomes, such as the morphology, the paticle chameter, and the protein content. The PBMC stimulated with anti-CD3/CD28 were incubated with the exosomes for five days, and then the percentage changes of Treg and TH17 cells were analyzed by using flow cytometry.
RESULTS
The hUC MSC-derived exosomes were saucer-like in morphology the averge diameter was approximately 142 nm. They were identified as positive for CD9 and CD63. Flow cytometry showed that the proportion of CD4CD25Foxp3 Treg cells in the PBMC were significantly higher, but the proportion of CD4IL17A T cells in the hUC-MSC-exosome group was obviously lower than that in the group without the hUC-MSC-exosom (control group) (P<0.05).
CONCLUSION
The hUC-MSC-exosomes have an immunomodulatory effect on T cells in vitro by increasing the ratio of Treg and reducing the ratio of TH17 cells, expecting the hUC-MSC-exosom as a novel cell-free target for immunotherapy.
目的
探讨人脐带间充质干细胞来源的外泌体对调节性T细胞（Treg）和辅助性T细胞17（TH17）细胞发育的影响。
方法
通过超速离心从人脐带间充质干细胞无血清培养上清液中收获外泌体。采用电子显微镜、纳米颗粒跟踪分析和蛋白质印迹法鉴定人脐带间充质干细胞来源的外泌体，包括形态、颗粒直径和蛋白质含量。将抗CD3/CD28刺激的外周血单个核细胞（PBMC）与外泌体共孵育5天，然后用流式细胞术分析Treg和TH17细胞的百分比变化。
结果
人脐带间充质干细胞来源的外泌体形态呈碟状，平均直径约为142nm。它们被鉴定为CD9和CD63阳性。流式细胞术显示，PBMC中CD4CD25Foxp3 Treg细胞的比例显著更高，但人脐带间充质干细胞外泌体组中CD4IL17A T细胞的比例明显低于无外泌体组（对照组）（P<0.05）。
结论
人脐带间充质干细胞来源的外泌体通过增加Treg比例和降低TH17细胞比例，在体外对T细胞具有免疫调节作用，有望将人脐带间充质干细胞外泌体作为一种新型的无细胞免疫治疗靶点。
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