Chen Xiao-Yu, Wang Shun-Qing
Department of Hematology，Guangzhou First People's Hospital，Guangzhou Medical University，Guangzhou 510180，Guangdong Province, China.
Department of Hematology，Guangzhou First People's Hospital，Guangzhou Medical University，Guangzhou 510180，Guangdong Province, China.E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Feb;27(1):239-245. doi: 10.7534/j.issn.1009-2137.2019.01.039.
To investigate the change of microbial diversity and its relation with gastrointestinal (GI) graft-versus-host disease (GVHD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT).
Fecal samples were collected at the time point of 2 weeks before transplantation (pre-transplant group), 1 month after transplantation (post-tranplant group) and onset of GI GVHD in 65 hematonosis patients, which were also collected in 26 donors and 10 healthy subjects (control group). 16S rRNA was extracted from fecal microbiotas whose V4 variable region was amplified. The amplification products were sequenced in Illumina HiSeq 2500 platform, and the sequencing results were analyzed and compared.
The microbial diversity was 5.70（3.74, 10.60）in pre-transplant group, 7.30（4.89, 11.41）in control group, and the differences between them were not statistically significant. The microbial diversity was 3.88（2.39, 6.49）in post-transplant group, lower than that in control group and pre-transplant group. After transplantation, the microbial diversity was 4.24（2.47, 7.16）in the patients without GI GVHD, while the microbial diverosity was 2.90 (1.48, 5.64) in patients subsequently suffered from GI GVHD, but the differences between them were not statistically significant. The microbial diversity was 2.13（1.76, 3.75）onset of GI GVHD, which was lower than that without GI GVHD.
Intestinal microbial diversity decreases after allo-HSCT, and is associated with with Gl GVHD.
探讨异基因造血干细胞移植（allo-HSCT）后微生物多样性的变化及其与胃肠道移植物抗宿主病（GVHD）的关系。
收集65例血液病患者移植前2周（移植前组）、移植后1个月（移植后组）及胃肠道GVHD发病时的粪便样本，同时收集26例供者和10例健康受试者的粪便样本作为对照组。从粪便微生物群中提取16S rRNA，扩增其V4可变区。扩增产物在Illumina HiSeq 2500平台上测序，并对测序结果进行分析比较。
移植前组微生物多样性为5.70（3.74，10.60），对照组为7.30（4.89，11.41），两组差异无统计学意义。移植后组微生物多样性为3.88（2.39，6.49），低于对照组和移植前组。移植后，未发生胃肠道GVHD的患者微生物多样性为4.24（2.47，7.16），随后发生胃肠道GVHD的患者微生物多样性为2.90（1.48，5.64），但两组差异无统计学意义。胃肠道GVHD发病时微生物多样性为2.13（1.76，3.75），低于未发生胃肠道GVHD者。
allo-HSCT后肠道微生物多样性降低，且与胃肠道GVHD有关。
