Second Department of Geriatrics, Aerospace Center Hospital, Beijing, China.
Department of Health Management, Aerospace Center Hospital, Beijing, China.
J Physiol Pharmacol. 2023 Feb;74(1). doi: 10.26402/jpp.2023.1.10. Epub 2023 May 23.
To observe the evolution of the intestinal microbiota in patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and discuss the relationship between the intestinal microbiota and graft-versus-host disease (GVHD). In this study, 11 patients who underwent allo-HSCT in the Aerospace Central Hospital from January 2021 to October 2021 were selected, along with 11 donors. Fecal specimens were collected 7 times: at admission, after pre-treatment, and every 3 weeks after transplantation from patients and once from donors. The composition of the intestinal microbiota and its association with GVHD after allogeneic hematopoietic stem cell transplantation were analyzed by 16S rRNA sequencing. Of the 11 patients, 5 developed GVHD, and 6 did not. The diversity of the intestinal microbiota among GVHD patients first increased and then decreased after transplantation, while that among non-GVHD patients first increased and then tended to be stable. The diversity of the intestinal microbiota among GVHD patients was lower than that among non-GVHD patients before pre-treatment and after transplantation. The taxa diversity of the intestinal microbiota in the non-GVHD group was better than that in the GVHD group before allo-HSCT, and the difference was statistically significant (P<0.05 for OTUs and CHAO1 index). The taxa abundance of Enterococcaceae 2.16% (2.13%, 2.22%) before allo-HSCT was significantly higher than that in the non-GVHD group 1.33% (0.27%, 1.52%), and the difference was statistically significant (P=0.004). There was no significant difference between the GVHD group and the non-GVHD group in the diversity of the intestinal microbiota of donors (P<0.05). The characteristics of the intestinal microbiota in the final sample of patients in the GVHD group were similar to the preoperative structure of the intestinal microbiota. In conclusion: The decrease in the diversity of the intestinal microbiota after HSCT may be a risk factor for the occurrence of GVHD. The presence of Enterococcaceae in the intestinal microbiota may be associated with an increased risk of developing GVHD. The intestinal microbiota reconstitute to be close to the intestinal microbiota composition of the donors in the non-GVHD group.
观察异基因造血干细胞移植（allo-HSCT）后患者肠道微生物群的演变，并探讨肠道微生物群与移植物抗宿主病（GVHD）之间的关系。本研究选择 2021 年 1 月至 2021 年 10 月在航天中心医院接受 allo-HSCT 的 11 例患者和 11 例供者，采集粪便标本：入院时、预处理后和移植后每 3 周采集患者标本，供者采集 1 次。采用 16S rRNA 测序分析肠道微生物群的组成及其与异基因造血干细胞移植后 GVHD 的关系。11 例患者中，5 例发生 GVHD，6 例未发生 GVHD。GVHD 患者肠道微生物群多样性在移植后先增加后减少，而非 GVHD 患者先增加后趋于稳定。预处理前和移植后，GVHD 患者的肠道微生物群多样性低于非 GVHD 患者。非 GVHD 组 allo-HSCT 前肠道微生物群的分类丰富度优于 GVHD 组，差异有统计学意义（OTUs 和 Chao1 指数 P<0.05）。allo-HSCT 前肠球菌科 2.16%（2.13%，2.22%）丰度明显高于非 GVHD 组 1.33%（0.27%，1.52%），差异有统计学意义（P=0.004）。GVHD 组和非 GVHD 组供者肠道微生物群多样性无差异（P<0.05）。GVHD 组患者最终样本的肠道微生物群特征与术前肠道微生物群结构相似。结论：HSCT 后肠道微生物群多样性的降低可能是 GVHD 发生的危险因素。肠道微生物群中肠球菌科的存在可能与发生 GVHD 的风险增加有关。非 GVHD 组肠道微生物群重建为接近供者的肠道微生物群组成。
