Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.).

A novel actinomycete, designated strain NEAU-HRGS1-13, was isolated from a root of wheat (Triticum aestivum L.) and characterised using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Microbispora. The major menaquinones were identified as MK-9(H) and MK-9(H) and the whole cell hydrolysates found to contain meso-diaminopimelic acid and madurose. The phospholipid profile was found to consist of diphosphatidylglycerol, a ninhydrin-positive glycophospholipid, phosphatidylinositol mannosides, phosphatidylmonomethylethanolamine, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as iso-C, C, 10-methyl C, C and C. The 16S rRNA gene sequence analysis showed that the isolate is closely related to Microbispora triticiradicis NEAU-HRDPA2-9 (99.4%), Microbispora bryophytorum NEAU-TX2-2 (99.0%), Microbispora camponoti 2C-HV3 (98.8%), Microbispora hainanensis DSM 45428 (98.8%), Microbispora amethystogenes JCM 3021 (98.6%), Microbispora siamensis NBRC 104113 (98.5%), Microbispora corallina JCM 10267 (98.3%) and Microbispora rosea subsp. rosea JCM 3006 (98.2%). However, DNA-DNA relatedness and cultural, physiological and biochemical data showed that strain NEAU-HRGS1-13 can be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-HRGS1-13 represents a novel species of the genus Microbispora, for which the name Microbispora tritici sp. nov. is proposed. The type stain is NEAU-HRGS1-13 (= CGMCC 4.7402 = DSM 104650).