Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Aug;112(8):1137-1145. doi: 10.1007/s10482-019-01246-y. Epub 2019 Feb 9.
A novel actinomycete, designated strain NEAU-HRGS1-13, was isolated from a root of wheat (Triticum aestivum L.) and characterised using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Microbispora. The major menaquinones were identified as MK-9(H) and MK-9(H) and the whole cell hydrolysates found to contain meso-diaminopimelic acid and madurose. The phospholipid profile was found to consist of diphosphatidylglycerol, a ninhydrin-positive glycophospholipid, phosphatidylinositol mannosides, phosphatidylmonomethylethanolamine, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as iso-C, C, 10-methyl C, C and C. The 16S rRNA gene sequence analysis showed that the isolate is closely related to Microbispora triticiradicis NEAU-HRDPA2-9 (99.4%), Microbispora bryophytorum NEAU-TX2-2 (99.0%), Microbispora camponoti 2C-HV3 (98.8%), Microbispora hainanensis DSM 45428 (98.8%), Microbispora amethystogenes JCM 3021 (98.6%), Microbispora siamensis NBRC 104113 (98.5%), Microbispora corallina JCM 10267 (98.3%) and Microbispora rosea subsp. rosea JCM 3006 (98.2%). However, DNA-DNA relatedness and cultural, physiological and biochemical data showed that strain NEAU-HRGS1-13 can be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-HRGS1-13 represents a novel species of the genus Microbispora, for which the name Microbispora tritici sp. nov. is proposed. The type stain is NEAU-HRGS1-13 (= CGMCC 4.7402 = DSM 104650).
一株新的放线菌，命名为 NEAU-HRGS1-13 菌株，从小麦（Triticum aestivum L.）根部分离得到，并采用多相分类方法进行了特征描述。形态学和化学分类学特征与 Microbispora 属的成员一致。主要的类异戊二烯醌为 MK-9(H) 和 MK-9(H)，全细胞水解产物中含有 meso-二氨基庚二酸和马杜糖。磷脂分析表明含有二磷脂酰甘油、一种水合茚三酮阳性糖脂、磷壁酸甘露糖苷、磷甲单乙醇胺、一种未鉴定的糖脂和一种未鉴定的脂类。主要脂肪酸为 iso-C、C、10-甲基 C、C 和 C。16S rRNA 基因序列分析表明，该分离株与小麦生微菌 NEAU-HRDPA2-9（99.4%）、石楠生微菌 NEAU-TX2-2（99.0%）、Camponoti 2C-HV3 微菌（98.8%）、海南生微菌 DSM 45428（98.8%）、紫黄质微菌 JCM 3021（98.6%）、暹罗生微菌 NBRC 104113（98.5%）、珊瑚生微菌 JCM 10267（98.3%）和玫瑰色微菌 subsp. rosea JCM 3006（98.2%）的相似度最高。然而，DNA-DNA 同源性以及培养、生理和生化数据表明，NEAU-HRGS1-13 菌株与亲缘关系较近的种可以区分开来。因此，认为 NEAU-HRGS1-13 菌株代表 Microbispora 属的一个新种，命名为小麦生微菌 Microbispora tritici sp. nov.。模式菌株为 NEAU-HRGS1-13（= CGMCC 4.7402 = DSM 104650）。
