Li Chuang, Zhang Yuejing, Liu Chongxi, Wang Haiyan, Zhao Junwei, Li Lianjie, Zhang Zhongwen, Wang Xiangjing, Xiang Wensheng
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1274-1279. doi: 10.1099/ijs.0.000095. Epub 2015 Jan 29.
A novel endophytic actinomycete, designated strain NEAU-TX2-2(T), was isolated from moss and characterized using a polyphasic approach. The isolate was found to have morphological characteristics typical of the genus Microbispora . The isolate formed longitudinally paired spores on the tips of short sporophores that branched from aerial hyphae. Analysis of the 16S rRNA gene sequence supported the assignment of the novel strain to the genus Microbispora , and strain NEAU-TX2-2(T) exhibited 99.08 and 98.62% gene sequence similarities to Microbispora amethystogenes JCM 3021(T) and Microbispora rosea subsp. rosea JCM 3006(T), respectively. However two tree-making algorithms supported the position that strain NEAU-TX2-2(T) formed a distinct clade with M. rosea subsp. rosea JCM 3006(T). A low level of DNA-DNA relatedness allowed the isolate to be differentiated from M. amethystogenes JCM 3021(T) and M. rosea subsp. rosea JCM 3006(T). Moreover, strain NEAU-TX2-2(T) could also be distinguished from its closest phylogenetic relatives by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TX2-2(T) represents a novel species of the genus Microbispora for which the name Microbispora bryophytorum sp. nov. is proposed. The type strain is NEAU-TX2-2(T) ( = CGMCC 4.7138(T) = DSM 46710(T)).
从苔藓中分离出一株新型内生放线菌，命名为菌株NEAU-TX2-2(T)，并采用多相分类法对其进行了表征。该分离株具有微小双孢菌属的典型形态特征。该分离株在从气生菌丝分支的短孢子梗顶端形成纵向成对的孢子。16S rRNA基因序列分析支持将该新菌株归入微小双孢菌属，菌株NEAU-TX2-2(T)与紫晶微小双孢菌JCM 3021(T)和玫瑰微小双孢菌玫瑰亚种JCM 3006(T)的基因序列相似性分别为99.08%和98.62%。然而，两种建树算法支持菌株NEAU-TX2-2(T)与玫瑰微小双孢菌玫瑰亚种JCM 3006(T)形成一个独特分支的位置。低水平的DNA-DNA相关性使该分离株能够与紫晶微小双孢菌JCM 3021(T)和玫瑰微小双孢菌玫瑰亚种JCM 3006(T)区分开来。此外，菌株NEAU-TX2-2(T)在形态和生理特征上也可与其最亲近的系统发育亲缘种相区分。因此，建议将菌株NEAU-TX2-2(T)代表微小双孢菌属的一个新物种，命名为苔藓微小双孢菌（Microbispora bryophytorum sp. nov.）。模式菌株为NEAU-TX2-2(T)（=CGMCC 4.7138(T)=DSM 46710(T)）。
