Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Clin Lab Anal. 2019 May;33(4):e22855. doi: 10.1002/jcla.22855. Epub 2019 Feb 9.
Empyema is one of the important causes of pediatric hospital admissions.
In this study, we had investigated the frequency rates of S aureus, S pneumoniae, H influenzae, and P aeruginosa using PCR and bacterial culture among children suffering from empyema in Ahvaz city, Iran.
This was a descriptive study conducted on the patients hospitalized in ICUs of two teaching Hospitals of Ahvaz, Iran, between March and September 2018 on 105 pleural fluid (PF) samples of the children less than 16 years of age with the diagnosis of empyema thoracis. These specimens were inoculated on the bacterial culture media and identified using biochemical characteristics. Then, the existence of the four pathogens mentioned above was evaluated using PCR method.
In this study, these bacteria agents were identified in 81 (77.14%) and 30 (28.57%) cases using the PCR assay and bacterial culture, respectively. Moreover, the PCR assay identified the infectious agents in 51 (68%) of PFs where the culture method failed. S pneumoniae (63 cases) was recognized as the most common pathogen, followed by P aeruginosa(19 cases), S aureus(15 cases), and H influenzae (9 cases) using the bacterial culture and PCR. Co-infections were detected in 21 samples (20%) using PCR and one sample using the bacterial culture (P aeruginosa and S pneumoniae).
In this study, we found the higher frequencies of these microorganisms using PCR than culture. In addition, we showed that PCR was a sensitive and accurate method that unaffected by antibiotic therapy and could detect well co-infections.
脓胸是小儿住院的重要原因之一。
本研究通过聚合酶链反应（PCR）和细菌培养检测金黄色葡萄球菌（S aureus）、肺炎链球菌（S pneumoniae）、流感嗜血杆菌（H influenzae）和铜绿假单胞菌（P aeruginosa）在伊朗阿瓦士市儿童脓胸患者中的频率。
这是一项描述性研究，于 2018 年 3 月至 9 月在伊朗阿瓦士两所教学医院的 ICU 对 105 例年龄小于 16 岁的脓胸患儿的 105 例胸腔积液（PF）样本进行，这些标本接种于细菌培养培养基上，并通过生化特征进行鉴定。然后，采用 PCR 法评估上述四种病原体的存在。
本研究中，PCR 法和细菌培养法分别在 81 例（77.14%）和 30 例（28.57%）患者中鉴定出这些细菌。此外，PCR 法在 51 例培养方法失败的 PF 中鉴定出感染因子。通过细菌培养和 PCR 法，肺炎链球菌（63 例）被认为是最常见的病原体，其次是铜绿假单胞菌（19 例）、金黄色葡萄球菌（15 例）和流感嗜血杆菌（9 例）。PCR 检测到 21 例（20%）混合感染，1 例（铜绿假单胞菌和肺炎链球菌）通过细菌培养检测到。
本研究发现 PCR 法检测这些微生物的频率高于培养法。此外，我们表明 PCR 是一种敏感和准确的方法，不受抗生素治疗的影响，可以很好地检测混合感染。
