Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.
PLoS One. 2013 Aug 28;8(8):e71774. doi: 10.1371/journal.pone.0071774. eCollection 2013.
An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients. P6-ELISA had a sensitivity of 83.3% for MEFs and 71.5% for NPSs and a specificity of 85.6% for MEFs and 92.5% for NPSs, respectively. Real-time PCR exhibited significant differences in the number of ompP1 gene copies among samples determined by P6-ELISA to be positive and negative for H. influenzae. However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly. Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.
酶联免疫吸附试验（ELISA）特异性针对外膜蛋白 P6（P6-ELISA），用于检测急性中耳炎（AOM）患者中耳液（MEF）和急性鼻-鼻窦炎（ARNS）患者鼻咽分泌物（NPS）中的流感嗜血杆菌。P6-ELISA 对 MEF 的敏感性为 83.3%，对 NPS 的敏感性为 71.5%，对 MEF 的特异性为 85.6%，对 NPS 的特异性为 92.5%。实时 PCR 显示，通过 P6-ELISA 确定为阳性和阴性的 H. influenzae 样本中 ompP1 基因拷贝数存在显著差异。然而，由于 P6-ELISA 试验在包括两种共生菌 H. haemolyticus 和 H. parainfluenzae 的嗜血杆菌属中具有反应性，因此它是一种弱方法，只能正确检测到 NTHi。因此，使用 P6-ELISA 的诊断应基于全面评估，包括其他相关检查和临床症状的结果，以防止在临床环境中得出误导性结论。
