一种针对外膜蛋白 p6 的酶联免疫吸附试验在检测中耳液和鼻咽分泌物中的流感嗜血杆菌的应用。
An application of outer membrane protein p6-specific enzyme-linked immunosorbent assay for detection of haemophilus influenzae in middle ear fluids and nasopharyngeal secretions.
机构信息
Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.
出版信息
PLoS One. 2013 Aug 28;8(8):e71774. doi: 10.1371/journal.pone.0071774. eCollection 2013.
An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients. P6-ELISA had a sensitivity of 83.3% for MEFs and 71.5% for NPSs and a specificity of 85.6% for MEFs and 92.5% for NPSs, respectively. Real-time PCR exhibited significant differences in the number of ompP1 gene copies among samples determined by P6-ELISA to be positive and negative for H. influenzae. However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly. Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.
酶联免疫吸附试验（ELISA）特异性针对外膜蛋白 P6（P6-ELISA），用于检测急性中耳炎（AOM）患者中耳液（MEF）和急性鼻-鼻窦炎（ARNS）患者鼻咽分泌物（NPS）中的流感嗜血杆菌。P6-ELISA 对 MEF 的敏感性为 83.3%，对 NPS 的敏感性为 71.5%，对 MEF 的特异性为 85.6%，对 NPS 的特异性为 92.5%。实时 PCR 显示，通过 P6-ELISA 确定为阳性和阴性的 H. influenzae 样本中 ompP1 基因拷贝数存在显著差异。然而，由于 P6-ELISA 试验在包括两种共生菌 H. haemolyticus 和 H. parainfluenzae 的嗜血杆菌属中具有反应性，因此它是一种弱方法，只能正确检测到 NTHi。因此，使用 P6-ELISA 的诊断应基于全面评估，包括其他相关检查和临床症状的结果，以防止在临床环境中得出误导性结论。
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