School of Biology and Biological Engineering , South China University of Technology , Guangzhou 510006 , People's Republic of China.
Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering , South China University of Technology , Guangzhou 510006 , People's Republic of China.
J Agric Food Chem. 2019 Mar 6;67(9):2570-2580. doi: 10.1021/acs.jafc.8b06382. Epub 2019 Feb 20.
A novel three-liquid-phase system which contained fish oil as the nonpolar phase was developed for the lipase-based hydrolysis of fish oil and subsequent enrichment of the omega-3 polyunsaturated fatty acids (n-3 PUFA) in the glyceride fraction of the fish oil. In comparison with the traditional oil/water system, the enrichment factor of n-3 PUFA in this system was increased by 363.4% as a result of a higher dispersity, higher selectivity of the lipase for the other fatty acids except for n-3PUFA, and relief of product inhibition. The content of n-3 PUFA in the glyceride fraction could be concentrated to 67.97% by repeated hydrolysis after removing the free fatty acids. Furthermore, the lipase could be reused for at least eight rounds. This method would be an ideal approach for enriching n-3 PUFA because it is cost-effective, low in toxicity, and easily scaled up.
开发了一种新型三相体系，其中包含鱼油作为非极性相，用于基于脂肪酶的鱼油水解和随后富集鱼油甘油酯部分中的ω-3 多不饱和脂肪酸 (n-3 PUFA)。与传统的油/水体系相比，由于分散性更高、脂肪酶对 n-3PUFA 以外的其他脂肪酸的选择性更高以及产物抑制作用的缓解，该体系中 n-3PUFA 的富集因子提高了 363.4%。除去游离脂肪酸后，通过重复水解可将甘油酯部分中的 n-3PUFA 含量浓缩至 67.97%。此外，脂肪酶至少可重复使用八轮。由于这种方法成本效益高、毒性低且易于放大，因此是一种理想的富集 n-3PUFA 的方法。
