Oliveira F L, Rumsey S C, Schlotzer E, Hansen I, Carpentier Y A, Deckelbaum R J
Department of Pediatrics, Columbia University, New York, NY 10032, USA.
JPEN J Parenter Enteral Nutr. 1997 Jul-Aug;21(4):224-9. doi: 10.1177/0148607197021004224.
Fish oil triglycerides (TG) are being considered for use in IV lipid emulsions, but the characteristics of their lipase-mediated clearance from plasma are largely unknown.
We compared the in vitro hydrolysis of soy oil long-chain triglyceride emulsions (LCT) and fish oil emulsions (omega-3) using lipoprotein (LPL) and hepatic (HL) lipases, omega-3 emulsions contained 18% and 28% of total TG fatty acid as eicosapentaenoic acid (EPA) and docosahexanoic acid (DHA), respectively.
Under conditions of maximal hydrolysis, total free fatty acid (FFA) release was two- to threefold greater with LCT compared with omega-3 emulsions. Also, EPA and DHA together contributed proportionally much less than other fatty acids (< 20%) to FFA released from omega-3 emulsions. In mixtures of LCT emulsion with omega-3 emulsions, the presence of > 20% of omega-3 particles substantially inhibited LCT emulsion hydrolysis (by up to 50%).
Our results suggest that, during infusion of omega-3 emulsions, EPA and DHA may enter cells as TG or partial glycerides within emulsion particles and not as FFA and that coinfusion of omega-3 emulsion with LCT emulsion at low omega-3:LCT emulsion ratios (up to 20% of total triglyceride as omega-3) will not substantially inhibit LCT hydrolysis.
鱼油甘油三酯（TG）正被考虑用于静脉脂质乳剂，但它们经脂肪酶介导从血浆中清除的特性在很大程度上尚不清楚。
我们使用脂蛋白脂肪酶（LPL）和肝脂肪酶（HL）比较了大豆油长链甘油三酯乳剂（LCT）和鱼油乳剂（ω-3）的体外水解情况，ω-3乳剂分别含有18%和28%的总TG脂肪酸为二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。
在最大水解条件下，与ω-3乳剂相比，LCT乳剂释放的总游离脂肪酸（FFA）多两到三倍。此外，EPA和DHA共同对从ω-3乳剂释放的FFA的贡献比例远低于其他脂肪酸（<20%）。在LCT乳剂与ω-3乳剂的混合物中，>20%的ω-3颗粒的存在显著抑制LCT乳剂的水解（高达50%）。
我们的结果表明，在输注ω-3乳剂期间，EPA和DHA可能作为乳剂颗粒内的TG或甘油部分进入细胞，而不是作为FFA，并且以低ω-3:LCT乳剂比例（高达总甘油三酯的20%为ω-3）将ω-3乳剂与LCT乳剂联合输注不会显著抑制LCT的水解。
