Campos J M, Sousa A C, Caseiro A R, Pedrosa S S, Pinto P O, Branquinho M V, Amorim I, Santos J D, Pereira T, Mendonça C M, Afonso A, Atayde L M, Maurício A C
Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, no 228, Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, Porto, Portugal.
Regen Biomater. 2019 Feb;6(1):49-59. doi: 10.1093/rb/rby025. Epub 2018 Dec 22.
Development of synthetic bone substitutes has arisen as a major research interest in the need to find an alternative to autologous bone grafts. Using an ovine model, the present pre-clinical study presents a synthetic bone graft (Bonelike) in combination with a cellular system as an alternative for the regeneration of non-critical defects. The association of biomaterials and cell-based therapies is a promising strategy for bone tissue engineering. Mesenchymal stem cells (MSCs) from human dental pulp have demonstrated both and to interact with diverse biomaterial systems and promote mineral deposition, aiming at the reconstruction of osseous defects. Moreover, these cells can be found and isolated from many species. Non-critical bone defects were treated with Bonelike with or without MSCs obtained from the human dental pulp. Results showed that Bonelike and MSCs treated defects showed improved bone regeneration compared with the defects treated with Bonelike alone. Also, it was observed that the biomaterial matrix was reabsorbed and gradually replaced by new bone during the healing process. We therefore propose this combination as an efficient binomial strategy that promotes bone growth and vascularization in non-critical bone defects.
由于需要找到自体骨移植的替代方法，合成骨替代物的开发已成为一项主要的研究兴趣点。本临床前研究使用绵羊模型，展示了一种合成骨移植材料（Bonelike）与细胞系统相结合，作为非关键性骨缺损再生的替代方法。生物材料与基于细胞的疗法相结合是骨组织工程的一种有前景的策略。来自人牙髓的间充质干细胞（MSCs）已显示出与多种生物材料系统相互作用并促进矿物质沉积的能力，旨在重建骨缺损。此外，这些细胞可以在许多物种中找到并分离出来。用Bonelike治疗非关键性骨缺损，添加或不添加从人牙髓获得的MSCs。结果表明，与单独用Bonelike治疗的缺损相比，用Bonelike和MSCs治疗的缺损显示出更好的骨再生。此外，观察到在愈合过程中生物材料基质被重新吸收并逐渐被新骨替代。因此，我们提出这种组合作为一种有效的二元策略，可促进非关键性骨缺损中的骨生长和血管形成。
