Chen Xiaoqian, Xu Peng, Zhou Jiawu, Tao Dayun, Yu Diqiu
Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Plant Divers. 2018 Sep 7;40(5):238-244. doi: 10.1016/j.pld.2018.09.001. eCollection 2018 Oct.
Plant height is an important trait related to yield potential and plant architecture. A suitable plant height plays a crucial role in improvement of rice yield and lodging resistance. In this study, we found that the traditional upland landrace 'Kaowenghan' (KWH) showed a special semi-dwarf phenotype. To identify the semi-dwarf gene from KWH, we raised BCF semi-dwarf introgression lines (IL) by hybridization of the rice cultivar 'Dianjingyou1' (DJY1) and KWH in a DJY1 background. The plant height of the homozygous semi-dwarf IL (IL-87) was significantly reduced compared with that of DJY1. The phenotype of the F progeny of the semi-dwarf IL-87 and DJY1 showed that the semi-dwarf phenotype was semi-dominant. QTL mapping indicated that the semi-dwarf phenotype was controlled by a major QTL and was localized between the markers RM6696 and RM12047 on chromosome 1. We also developed near-isogenic lines (NIL) from the BCF population, and found that the yield of homozygous NIL (NIL-2) was not significantly different compared to DJY1. Breeding value evaluation through investigation of the plant height of the progeny of NIL (NIL-2) and cultivars from different genetic background indicate that the novel semi-dwarf gene shows potential as a genetic resource for rice breeding.
株高是与产量潜力和株型相关的重要性状。合适的株高对提高水稻产量和抗倒伏能力起着关键作用。在本研究中，我们发现传统旱稻地方品种‘考文汉’（KWH）表现出特殊的半矮化表型。为了从KWH中鉴定半矮化基因，我们以水稻品种‘滇粳优1号’（DJY1）为背景，通过DJY1与KWH杂交培育了BCF半矮化导入系（IL）。与DJY1相比，纯合半矮化IL（IL - 87）的株高显著降低。半矮化IL - 87与DJY1的F子代表型表明，半矮化表型为半显性。QTL定位表明，半矮化表型受一个主QTL控制，定位于第1染色体上的标记RM6696和RM12047之间。我们还从BCF群体中培育了近等基因系（NIL），发现纯合NIL（NIL - 2）的产量与DJY1相比无显著差异。通过调查NIL（NIL - 2）与不同遗传背景品种的子代株高进行育种值评估表明，该新型半矮化基因具有作为水稻育种遗传资源的潜力。
