Wennogle S A, Randall E K, Priestnall S L, Twedt D C, Simpson K W
Department of Clinical Sciences, College of Veterinary Medicine, Colorado State University, Fort Collins, Colorado 80523, USA.
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
J Small Anim Pract. 2019 May;60(5):291-297. doi: 10.1111/jsap.12982. Epub 2019 Feb 10.
To detect and localise bacteria in gallbladder mucoceles using fluorescence in situ hybridisation (FISH). To report clinical signs, clinicopathologic abnormalities, sonographic findings and histopathological findings in FISH+ and FISH- dogs with gallbladder mucoceles.
Retrospective review of signalment, clinical signs, clinicopathologic and sonographic findings of 25 cases of histopathologically confirmed gallbladder mucocele. Histopathological sections of gallbladder mucocele were evaluated for cystic mucinous hyperplasia, cystic mucinous hyperplasia with cholecystitis and rupture. The number and spatial distribution of bacteria was determined by eubacterial FISH. Gallbladder contents were cultured in 21 dogs.
Bacteria were detected within or adherent to the gallbladder wall in eight of 25 (32%) cases. Bacterial culture was positive in one dog. Cystic mucinous hyperplasia with concurrent cholecystitis was found in 17 of 25 (68%) of dogs with gallbladder mucocele.
FISH was more sensitive for detection of bacteria in gallbladder mucoceles when compared to bacterial culture of bile. Cholecystitis was common in dogs with gallbladder mucocele. Further study is required to elucidate the relationship of cystic mucinous hyperplasia, bacteria and cholecystitis in the aetiopathogenesis and progression of gallbladder mucocele.
使用荧光原位杂交（FISH）检测胆囊黏液囊肿中的细菌并对其进行定位。报告FISH阳性和FISH阴性的胆囊黏液囊肿犬的临床症状、临床病理异常、超声检查结果和组织病理学结果。
回顾性分析25例经组织病理学确诊的胆囊黏液囊肿病例的信号、临床症状、临床病理和超声检查结果。对胆囊黏液囊肿的组织病理学切片进行囊性黏液性增生、伴有胆囊炎的囊性黏液性增生和破裂的评估。通过真细菌FISH确定细菌的数量和空间分布。对21只犬的胆囊内容物进行培养。
25例中有8例（32%）在胆囊壁内或附着于胆囊壁处检测到细菌。1只犬的细菌培养呈阳性。25只患有胆囊黏液囊肿的犬中有17只（68%）发现伴有胆囊炎的囊性黏液性增生。
与胆汁细菌培养相比，FISH对胆囊黏液囊肿中细菌的检测更敏感。胆囊炎在患有胆囊黏液囊肿的犬中很常见。需要进一步研究以阐明囊性黏液性增生、细菌和胆囊炎在胆囊黏液囊肿病因学发病机制和进展中的关系。
