School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, China.
School of Basic Medicine and Biological Sciences, Soochow University, Sericulture Institute of Soochow University, Suzhou, China.
Pest Manag Sci. 2019 Oct;75(10):2672-2681. doi: 10.1002/ps.5373. Epub 2019 Mar 14.
Neonicotinoid insecticides are widely used in the prevention and control of pests in agriculture and forestry, but they can also affect the development of nontarget economic insects. In order to determine the effects of trace acetamiprid on the development of reproductive system of silkworm, we studied the gonad development and endogenous hormone metabolism in silkworms exposed to trace acetamiprid.
The silkworm showed mild poisoning symptoms after being exposed to trace acetamiprid (0.01 mg L ). After 96 h of acetamiprid exposure, the body weight of silkworm was decreased by 7.67% and the ovary and fallopian tube were abnormally developed. The egg production in adults was decreased by 197 ± 29, and the egg weight was decreased by 0.52 ± 0.01 mg. Quantitative RT-PCR analysis showed that the relative expression levels of ovarian development-related genes Vg, Ovo, Otu, Sxl-S and Sxl-L were downregulated by 0.71, 0.77, 0.47, 0.67 and 0.88-fold, respectively. The transcriptional expression of ecdysone metabolism-related gene (EcR) in the ovary was downregulated by 0.46-fold while the expression of juvenile hormone-related gene (JHBP2) was upregulated by 1.36-fold. In hemolymph of acetamiprid exposed larvae, the content of ecdysone was reduced whereas the content of juvenile hormone was increased.
Trace acetamiprid may cause reproductive dysfunction in silkworms through regulating the metabolism of endogenous hormones. Our study provides a reference for elucidating the mechanism of acetamiprid-induced reproductive disorders in insects. © 2019 Society of Chemical Industry.
新烟碱类杀虫剂在农林害虫防治中被广泛使用，但也会影响非靶标经济昆虫的发育。为了确定微量啶虫脒对家蚕生殖系统发育的影响，我们研究了暴露于微量啶虫脒的家蚕的性腺发育和内源性激素代谢。
家蚕在暴露于微量啶虫脒（0.01mg/L）后表现出轻微中毒症状。在啶虫脒暴露 96 小时后，家蚕体重下降 7.67%，卵巢和输卵管发育异常。成虫产卵量减少 197±29 个，卵重减少 0.52±0.01mg。定量 RT-PCR 分析显示，卵巢发育相关基因 Vg、Ovo、Otu、Sxl-S 和 Sxl-L 的相对表达水平分别下调了 0.71、0.77、0.47、0.67 和 0.88 倍。卵巢中蜕皮激素代谢相关基因（EcR）的转录表达下调了 0.46 倍，而保幼激素相关基因（JHBP2）的表达上调了 1.36 倍。在啶虫脒暴露幼虫的血淋巴中，蜕皮激素的含量减少，而保幼激素的含量增加。
微量啶虫脒可能通过调节内源性激素代谢引起家蚕生殖功能障碍。我们的研究为阐明啶虫脒诱导昆虫生殖障碍的机制提供了参考。© 2019 英国化学学会。
