School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, People's Republic of China.
Sericulture Institute of Soochow University, Suzhou, Jiangsu, People's Republic of China.
Environ Toxicol. 2019 Sep;34(9):1043-1051. doi: 10.1002/tox.22775. Epub 2019 May 23.
Acetamiprid is widely used for agricultural pest control. However, it remains poorly understood whether the environmental residues of acetamiprid have the potential effects on economic insect. In this study, we evaluated the effects of acetamiprid on silkworm growth and development. The exposure to trace amounts of acetamiprid significantly decreased body weight, viability, and spinning ability. In addition, the activity of trypsin in the midgut was decreased after exposure. DGE and KEGG pathway enrichment analysis revealed that the significantly differentially expressed genes were mainly involved in nutrient metabolism, stress responses, and inflammation pathways. These results, in combination with hematoxylin-eosin staining and transmission electron microscopy, indicated that acetamiprid could cause oxidative damage to midgut, lead to inflammatory responses, and affect the activities of midgut digestive enzymes, thus resulting in abnormal growth and development. Our findings greatly contributed to the evaluation of the effects of acetamiprid residues on other nontarget beneficial insect.
乙酰甲胺磷被广泛用于农业害虫防治。然而，环境残留的乙酰甲胺磷是否对经济昆虫具有潜在影响仍知之甚少。在本研究中，我们评估了乙酰甲胺磷对家蚕生长发育的影响。微量暴露于乙酰甲胺磷会显著降低蚕的体重、存活率和吐丝能力。此外，暴露后中肠的胰蛋白酶活性降低。DGE 和 KEGG 途径富集分析表明，差异表达基因主要参与营养代谢、应激反应和炎症途径。这些结果结合苏木精-伊红染色和透射电子显微镜观察，表明乙酰甲胺磷可引起中肠氧化损伤，导致炎症反应，并影响中肠消化酶的活性，从而导致异常生长发育。我们的研究结果对评估乙酰甲胺磷残留对其他非靶标有益昆虫的影响具有重要意义。
