a Department of Marine-Bio Convergence Science , Pukyong National University , Busan , Republic of Korea.
b Food Safety and Processing Research Division , National Fisheries Research & Development Institute , Busan , Republic of Korea.
Nutr Cancer. 2019;71(1):118-127. doi: 10.1080/01635581.2018.1540717. Epub 2019 Feb 9.
Recent evidence provides that seafood has a lot of health benefits due to its unique bioactive compounds. Sea squirt is widely cultured and consumed as a foodstuff in Korea; however, seldom reports with reference to bioactivities are available until now. In this study, edible part of sea squirt was hydrolyzed by pepsin and its hydrolysates was evaluated for anticancer effect on human colon cancer HT-29 cells. Sea squirt hydrolysates (SSQ) reduced HT-29 cell viability. Treatment with SSQ resulted in the increase in reactive oxygen species (ROS) generation followed by disruption of mitochondrial membrane potential (MMP). Flow cytometry analysis revealed that SSQ induced G2/M phase arrest and apoptosis evidenced by Hoechst 33342 staining. Levels of mRNA expression by real-time polymerase chain reaction (PCR) showed that treatment with SSQ in HT-29 cells upregulated expression of p53, bax, and caspase-3 genes and downregulated expression of bcl-2 gene. Protein level of cytochrome c into cytosol and caspase-3 by Western blotting were also increased by treatment with SSQ in HT-29 cells. These results suggest that SSQ may be useful for functional food ingredients and/or nutraceuticals.
最近的证据表明，由于其独特的生物活性化合物，海鲜对人体健康有很多益处。海鞘在韩国被广泛养殖和食用，但到目前为止，很少有关于其生物活性的报道。在这项研究中，海鞘的可食用部分用胃蛋白酶水解，然后评估其对人结肠癌细胞 HT-29 的抗癌作用。海鞘水解物（SSQ）降低了 HT-29 细胞活力。用 SSQ 处理会导致活性氧（ROS）生成增加，随后线粒体膜电位（MMP）破坏。流式细胞术分析显示，SSQ 通过 Hoechst 33342 染色诱导 G2/M 期阻滞和凋亡。实时聚合酶链反应（PCR）的 mRNA 表达水平表明，SSQ 处理 HT-29 细胞上调了 p53、bax 和 caspase-3 基因的表达，下调了 bcl-2 基因的表达。用 SSQ 处理 HT-29 细胞后，细胞质中的细胞色素 c 和 caspase-3 的蛋白水平也增加了。这些结果表明，SSQ 可能是功能性食品成分和/或营养保健品的有用来源。
