Thorne G M
Harvard Medical School, Boston, Massachusetts.
Infect Dis Clin North Am. 1988 Sep;2(3):747-74.
For many years, microbiologic examination of feces was focused on the isolation of two members of the family Enterobacteriaceae--Salmonellae and Shigellae. Over the past two decades, other enteric pathogens such as the various classes of diarrheagenic E. coli, Campylobacter, Vibrio spp., Yersinia enterocolitica, and Clostridium difficile have gained prominence. A newly recognized protozoan parasite, Cryptosporidium is now known to infect both immunocompetent and immunodeficient individuals. Added to these is the growing list of viruses incriminated in diarrheal diseases and gastritis. This article provides some of the basic information needed to allow for the isolation and identification of many of the currently recognized enteric pathogens. It also provides information on some of the currently available rapid test procedures that will speed up stool specimen testing and allow for more timely reporting to the physician.
多年来,粪便的微生物学检查主要集中于分离肠杆菌科的两个成员——沙门氏菌和志贺氏菌。在过去二十年中,其他肠道病原体,如各类致泻性大肠杆菌、弯曲杆菌、弧菌属、小肠结肠炎耶尔森氏菌和艰难梭菌等,已日益受到关注。一种新发现的原生动物寄生虫——隐孢子虫,现已明确可感染免疫功能正常和免疫功能低下的个体。此外,导致腹泻病和胃炎的病毒种类也越来越多。本文提供了一些基本信息,有助于分离和鉴定目前公认的许多肠道病原体。还介绍了一些现有的快速检测方法,这些方法可加快粪便标本检测速度,并能更及时地向医生报告结果。