基于金纳米粒子的催化组装和计数的无扩增超灵敏蛋白激酶检测。
Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles.
机构信息
Department of Chemistry, Shanghai Stomatological Hospital, and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, 200433, China.
出版信息
Chem Commun (Camb). 2019 Feb 21;55(17):2505-2508. doi: 10.1039/c9cc00131j.
PMID:30741307
Abstract
A single-particle enumeration method based on phosphorylation-directed in situ assembly of gold nanoparticles is developed for the ultrasensitive sensing of cellular protein kinase A activity. In comparison to existing strategies, the proposed new method demonstrates five orders of linear range and improves the detection limit up to 10-to-1000 fold without the involvement of target amplification.
摘要
基于金纳米粒子的磷酸化导向原位组装的单粒子计数方法被开发出来，用于超灵敏检测细胞蛋白激酶 A 的活性。与现有策略相比，所提出的新方法在不涉及目标放大的情况下，将线性范围提高了五个数量级，检测限提高了 10 到 1000 倍。
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