Ihermann-Hella Anneliis, Kuure Satu
GM-unit at Helsinki Institute of Life Science and Medicum, University of Helsinki, Helsinki, Finland.
GM-unit at Helsinki Institute of Life Science and Medicum, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Methods Mol Biol. 2019;1926:23-30. doi: 10.1007/978-1-4939-9021-4_2.
Kidney organogenesis has been a widely used classical model system to study inductive tissue interactions that guide differentiation of many organs. The basis for this is in the pioneering work done during the early 1950s when the conditions of how to support ex vivo growth and differentiation of developing kidneys were revealed. Importantly, culturing developing kidneys remains as an essential instrument to advance our understanding of molecular and cellular regulation of morphogenesis even today. Despite the fact that embryonic kidneys have been cultured for decades, it is not a trivial method and requires specific anatomical and developmental biology knowledge. This chapter outlines the general steps in organ culture and details the requirements for successful kidney explant differentiation.
肾脏器官发生一直是一个广泛使用的经典模型系统，用于研究指导许多器官分化的诱导性组织相互作用。其依据是20世纪50年代早期所做的开创性工作，当时揭示了支持发育中肾脏体外生长和分化的条件。重要的是，即使在今天，培养发育中的肾脏仍然是增进我们对形态发生的分子和细胞调节理解的重要手段。尽管胚胎肾脏已经被培养了几十年，但这并不是一种简单的方法，需要特定的解剖学和发育生物学知识。本章概述了器官培养的一般步骤，并详细说明了成功进行肾脏外植体分化的要求。
