Brown Aaron C, Gupta Ashwani K, Oxburgh Leif
Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME, USA.
Methods Mol Biol. 2019;1926:63-75. doi: 10.1007/978-1-4939-9021-4_6.
Nephrons differentiate from the cap mesenchyme of the fetal kidney. Nephron progenitor cells that populate the cap mesenchyme efficiently balance self-renewal and epithelial differentiation to enable repeated rounds of nephron formation during development. Here we describe a method to isolate and propagate these cells from the embryonic mouse kidney. Using this method, nephron progenitor cells from a single litter of mice can be propagated to hundreds of millions of cells that express appropriate markers of the undifferentiated state and retain epithelial differentiation capacity in vitro.
肾单位由胎儿肾脏的帽状间充质分化而来。定位于帽状间充质的肾单位祖细胞有效地平衡自我更新和上皮分化，从而在发育过程中实现多轮肾单位形成。在此，我们描述一种从胚胎小鼠肾脏中分离和扩增这些细胞的方法。使用该方法，来自一窝小鼠的肾单位祖细胞可扩增至数亿个细胞，这些细胞表达未分化状态的合适标志物，并在体外保留上皮分化能力。
