从小鼠和人类多能干细胞生成三维肾单位

Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells.

作者信息

Yoshimura Yasuhiro, Taguchi Atsuhiro, Nishinakamura Ryuichi

机构信息

Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.

Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany.

出版信息

Methods Mol Biol. 2019;1926:87-102. doi: 10.1007/978-1-4939-9021-4_8.

DOI:10.1007/978-1-4939-9021-4_8

PMID:30742265

Abstract

Nephrons, the functional units of the kidney, are derived from nephron progenitor cells (NPCs). Here, we describe methods to reconstruct nephron tissue via induction of NPCs from mouse and human pluripotent stem cells, which mimic multistep developmental signals in vivo. Induced NPCs differentiate into three-dimensional nephron structures, including glomerular podocytes and nephric tubules, which are useful for studying early stages of kidney specification and morphogenetic processes in the context of normal development or disease.

摘要

肾单位作为肾脏的功能单位，由肾单位祖细胞（NPCs）分化而来。在此，我们描述了从小鼠和人类多能干细胞诱导产生NPCs以重建肾单位组织的方法，这些方法模拟了体内多步骤的发育信号。诱导产生的NPCs可分化为三维肾单位结构，包括肾小球足细胞和肾小管，这对于在正常发育或疾病背景下研究肾脏特化的早期阶段和形态发生过程非常有用。