Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture.
作者信息
Lawrence Melanie L, Elhendawi Mona, Davies Jamie A
机构信息
Deanery of Biomedical Sciences, University of Edinburgh, Edinburgh, UK.
Faculty of Medicine, Clinical Pathology Department, Mansoura University, El-Mansoura, Egypt.
出版信息
Methods Mol Biol. 2019;1926:127-142. doi: 10.1007/978-1-4939-9021-4_11.
Some aspects of renal physiology, in particular transport across tubular epithelia, are highly relevant to pharmacokinetics and to drug toxicity. The use of animals to model human renal physiology is limited, but human-derived renal organoids offer an alternative, relevant system in culture. Here, we explain how the activity of specific transport systems can be assessed in renal organoid and organ culture, using a system illustrated mainly for mouse but that can be extended to human organoids.
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