Akhtar Muhammad Faheem, Wei Quanwei, Zhu Huanxi, Chen Zhe, Ahmad Ejaz, Zhendan Shi, Shi Fangxiong
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Theriogenology. 2019 Apr 1;128:122-132. doi: 10.1016/j.theriogenology.2019.01.039. Epub 2019 Feb 2.
The present study was designed to investigate the potential role of immunization against inhibin on testicular development, plasma testosterone concentration and expression of relevant genes in hypothalamus, pituitary, Leydig and Sertoli cells in Yangzhou ganders. For this purpose, Yangzhou ganders, n = 30 were divided into groups A and B. Group B ganders were actively immunized against inhibin α-subunit, while group A ganders were immunized with bovine serum albumin (BSA), which served as control. Immunization against inhibin elevated testes weights. In addition, immunization against inhibin elevated GnRH, StAR, CYP11A1 and AMH mRNA transcription expressions as depicted by qRT-PCR. Furthermore, hypothalamic GnRH-I mRNA expressions were up regulated, while GnIH mRNA transcription expression showed reciprocal expression on day 227. LH-β mRNA transcription expression remained unaffected. In conclusion, our findings suggest that active immunization against inhibin affect spermatogenesis and testicular development through regulations of hypothalamic, pituitary and testicular genes expressions.
本研究旨在探讨免疫抑制素对扬州公鹅睾丸发育、血浆睾酮浓度以及下丘脑、垂体、睾丸间质细胞和支持细胞中相关基因表达的潜在作用。为此，将30只扬州公鹅分为A组和B组。B组公鹅主动免疫抑制素α亚基，而A组公鹅用牛血清白蛋白（BSA）免疫，作为对照。免疫抑制素可提高睾丸重量。此外，qRT-PCR结果显示，免疫抑制素可提高GnRH、StAR、CYP11A1和AMH mRNA转录表达。此外，下丘脑GnRH-I mRNA表达上调，而GnIH mRNA转录表达在第227天呈相反表达。LH-β mRNA转录表达未受影响。总之，我们的研究结果表明，主动免疫抑制素通过调节下丘脑、垂体和睾丸基因表达来影响精子发生和睾丸发育。
