Meng Jinzhu, Feng Jianhao, Xiao Lilin, Hu Nan, Lan Xianyong, Wang Shuilian
College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren, China.
Anim Reprod. 2024 Oct 4;21(4):e20230079. doi: 10.1590/1984-3143-AR2023-0079. eCollection 2024.
The objective of the present study was to evaluate the effects of a novel Inhibin (INH) DNA vaccine (C500/pVAX-asd-IS) on the immune response, reproductive hormone levels, and spermatogenesis of rats. Forty healthy male rats were divided into four groups, and respectively immunized (thrice, 14 d apart) with 1×10, 1×10, and 1×10 CFU of the recombinant inhibin vaccine (group C500/pVAX-asd-IS-L, C500/pVAX-asd-IS-M, and C500/pVAX-asd-IS-H) or 1×10 CFU C500. P/N values increased after vaccination and differed ( <0.05) at 7 d, and sharply increased at 14 d following the booster vaccination ( <0.01); The weight and volume of testes in C500/pVAX-asd-IS groups were increased ( < 0.05) at decapitation, respectively; Histological evaluation showed that the number of spermatogenic cells in the lumen was increased, and the cytoplasmic remnants of sperms were allergy increased significantly compared with the control group. Oral vaccination with INH DNA reduced ( < 0.05) serum concentrations of INH B, enhanced serum concentrations of testosterone (T) and FSH. Furthermore, mRNA expressions of and in the testes were increased in C500/pVAX-asd-IS-M and C500/pVAX-asd-IS-H groups ( < 0.05 or < 0.01). The mRNA amount of in C500/pVAX-asd-IS-M group was greater than control group ( < 0.05).These results suggested that neutralization of endogenous INH through oral vaccination with INH DNA delivered by C500 strain successfully elicited a humoral immune response. INH gene immunization may have a positive effect on spermatogenesis and reproductive efficiency in male rats.
本研究的目的是评估一种新型抑制素（INH）DNA疫苗（C500/pVAX-asd-IS）对大鼠免疫反应、生殖激素水平和精子发生的影响。将40只健康雄性大鼠分为四组，分别用1×10、1×10和1×10 CFU的重组抑制素疫苗（C500/pVAX-asd-IS-L组、C500/pVAX-asd-IS-M组和C500/pVAX-asd-IS-H组）或1×10 CFU C500进行免疫（共三次，间隔14天）。接种疫苗后P/N值升高，在第7天有所差异（<0.05），加强免疫后第14天急剧升高（<0.01）；处死时，C500/pVAX-asd-IS组睾丸的重量和体积分别增加（<0.05）；组织学评估显示，与对照组相比，管腔内生精细胞数量增加，精子的细胞质残余物明显增多。口服INH DNA疫苗可降低（<0.05）血清中INH B的浓度，提高血清睾酮（T）和促卵泡激素（FSH）的浓度。此外，C500/pVAX-asd-IS-M组和C500/pVAX-asd-IS-H组睾丸中 和 的mRNA表达增加（<0.05或<0.01）。C500/pVAX-asd-IS-M组 的mRNA量大于对照组（<0.05）。这些结果表明，通过口服由C500菌株递送的INH DNA疫苗中和内源性INH可成功引发体液免疫反应。INH基因免疫可能对雄性大鼠的精子发生和生殖效率有积极影响。
