Azorín-Vega Erika, Aranda-Lara Liliana, Torres-García Eugenio, Santiago-Bañuelos Juan Antonio
Departamento de Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, 52750, Estado de México, Mexico.
Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, 50180, Estado de México, Mexico.
Appl Radiat Isot. 2019 Apr;146:24-28. doi: 10.1016/j.apradiso.2019.01.021. Epub 2019 Jan 28.
The therapeutic potential of Lu-iPSMA on hypoxic cancer cells has not been yet demonstrated. The aim of this work was to evaluate the radiation dose effect of Lu-iPSMA on viability and DNA damage in U87MG human glioma cells subjected to hypoxia-mimetic conditions. U87MG cells treated with Lu-iPSMA were incubated with CoCl in order to induce hypoxia-mimetic conditions. The cytotoxic and genotoxic effect was evaluated with an in vitro viability test and a neutral comet assay. Lu-iPSMA decreased the cell viability and induced DNA double strand breaks in U87MG human glioma cells under hypoxia-mimetic conditions. Lu-iPSMA produced the maximum effect at 48 h, suggesting that this radiopharmaceutical could be used as a strategy for the treatment of human glioma hypoxic cells.
Lu-iPSMA对缺氧癌细胞的治疗潜力尚未得到证实。这项工作的目的是评估Lu-iPSMA在模拟缺氧条件下对U87MG人胶质瘤细胞活力和DNA损伤的辐射剂量效应。用Lu-iPSMA处理的U87MG细胞与CoCl孵育以诱导模拟缺氧条件。通过体外活力试验和中性彗星试验评估细胞毒性和遗传毒性效应。在模拟缺氧条件下，Lu-iPSMA降低了U87MG人胶质瘤细胞的活力并诱导了DNA双链断裂。Lu-iPSMA在48小时产生最大效应，表明这种放射性药物可作为治疗人胶质瘤缺氧细胞的一种策略。
