Glasa Miroslav, Bananej Kaveh, Predajňa Lukáš, Vahdat Aisan
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
Plant Virus Research Department, Iranian Research Institute of Plant Protection (IRIPP), Tehran, Iran.
Plant Dis. 2011 Jan;95(1):38-42. doi: 10.1094/PDIS-05-10-0355.
Although Watermelon mosaic virus (WMV) is one of the main cucurbit pathogens and has a worldwide distribution, reliable data on its molecular variability is still limited to some geographical regions. The genetic diversity of 36 WMV isolates from Slovakia and Iran were studied by sequence analysis targeting two opposite genomic regions (P1 and NIb-CP). Phylogenetic analysis using partial sequences of the P1 gene showed that Slovak WMV isolates had greater diversity, representing two groups (group 1 and group 2), whereas all Iranian isolates belonged to a single group (group 2), with relatively low divergeance. Interestingly, in the NIb-CP region, all analyzed Slovak and Iranian isolates clustered within the group 1, thereby illustrating the phylogenetic discrepancies between the two analyzed genomic regions. Based on these data, one-half of analyzed Slovak isolates and all Iranian WMV isolates showed a switch in affiliation based on considered genomic region, clearly indicating their recombinant nature. This work provides further evidence of the significant contribution of recombination to the evolutionary history of WMV and outlines the necessity to target more than a single genome fragment for accurate typing of WMV isolates.
尽管西瓜花叶病毒（WMV）是主要的葫芦科病原体之一，且在全球范围内分布，但关于其分子变异性的可靠数据仍仅限于某些地理区域。通过对来自斯洛伐克和伊朗的36株WMV分离株的两个相对基因组区域（P1和NIb-CP）进行序列分析，研究了它们的遗传多样性。使用P1基因部分序列进行的系统发育分析表明，斯洛伐克的WMV分离株具有更大的多样性，代表两个组（第1组和第2组），而所有伊朗分离株都属于一个单一的组（第2组），差异相对较小。有趣的是，在NIb-CP区域，所有分析的斯洛伐克和伊朗分离株都聚集在第1组内，从而说明了两个分析的基因组区域之间的系统发育差异。基于这些数据，根据所考虑的基因组区域，一半的分析斯洛伐克分离株和所有伊朗WMV分离株显示出归属的转变，清楚地表明了它们的重组性质。这项工作进一步证明了重组对WMV进化史的重大贡献，并概述了针对多个基因组片段对WMV分离株进行准确分型的必要性。
