Verma Rakesh Kumar, Mishra Megha, Marwal Avinash, Gaur R K
1School of Liberal Arts and Sciences, Mody University, Lakshmangarh, Sikar, Rajasthan 332311 India.
2Department of Biotechnology, Mohanlal Sukhadia University, Vigyan Bhawan-Block B, New Campus, Udaipur, Rajasthan 313001 India.
3 Biotech. 2020 Jun;10(6):257. doi: 10.1007/s13205-020-02248-8. Epub 2020 May 17.
(WMV) is an important virus causing adverse effects on cucurbits throughout the world. In this study, we recorded WMV infection in the watermelon (-growing area of Alwar and Sikar in districts of Rajasthan, India. The RT-PCR-based detection was performed to confirm the presence of WMV, by using potyvirus-degenerated coat protein primers. Further, the complete genome sequences of two WMV isolates were compared with previously reported genome sequences. The complete genome of each isolate was 10,030 nt long, excluding the poly-A tails. Phylogeny relationships of the WMV isolates in the present study revealed the presence of uneven evolutionary pressure among the different WMV viral genomic segments. The analysis revealed that all the WMV isolates were divided into three clusters and the Indian WMV isolates cluster together with the French isolate. Recombination analysis of WMV exhibited significant recombination hotspots in the P1, NIa-Pro and Nib-CP regions. Our finding highlights the importance of genetic variability and recombination analysis to provide a better understanding of WMV molecular diversity.
西瓜花叶病毒（WMV）是一种对全球葫芦科作物产生不利影响的重要病毒。在本研究中，我们记录了印度拉贾斯坦邦阿尔瓦尔和锡卡尔地区西瓜种植区的WMV感染情况。通过使用马铃薯Y病毒属的外壳蛋白简并引物，进行基于逆转录聚合酶链反应（RT-PCR）的检测以确认WMV的存在。此外，将两个WMV分离株的完整基因组序列与先前报道的基因组序列进行了比较。每个分离株的完整基因组长度为10,030个核苷酸，不包括多聚腺苷酸尾巴。本研究中WMV分离株的系统发育关系表明，不同WMV病毒基因组片段之间存在不均衡的进化压力。分析表明，所有WMV分离株分为三个簇，印度WMV分离株与法国分离株聚集在一起。WMV的重组分析显示，在P1、NIa-Pro和Nib-CP区域存在显著的重组热点。我们的研究结果突出了遗传变异性和重组分析对于更好地理解WMV分子多样性的重要性。
