Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7, Canada.
Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, 1 Airport Road, Swift Current, SK S9H 3X2, Canada.
Sci Total Environ. 2019 Apr 10;660:1135-1143. doi: 10.1016/j.scitotenv.2019.01.100. Epub 2019 Jan 11.
While establishment and persistence of arbuscular mycorrhizal (AM) fungal inoculants in agricultural fields are critical to their success, little is known about how farming practices can affect their establishment in field. We developed a probe assay specific to a commercial AM fungal inoculant (Rhizoglomus irregulare DAOM197198) and tested its establishment among different grain cropping practices in the field. Establishment of the fungus was not related to cropping, or inoculation practices. Instead, establishment was site specific over the two growing seasons. Our results show that it is not yet possible to predict inoculation success in the field and use of biofertilizers requires further research under field conditions to identify key factors involved in establishment and persistence.
虽然丛枝菌根（AM）真菌接种体在农业领域的建立和持续存在对于其成功至关重要，但对于耕作实践如何影响其在田间的建立，人们知之甚少。我们开发了一种针对商业 AM 真菌接种体（Rhizoglomus irregulare DAOM197198）的探针检测方法，并在田间不同的谷物种植实践中测试了其建立情况。真菌的建立与种植或接种实践无关。相反，在两个生长季节中，建立是特定于地点的。我们的结果表明，目前尚不可能预测田间接种的成功，并且需要在田间条件下进一步研究生物肥料的使用，以确定建立和持续存在涉及的关键因素。
