Department of Biological and Chemical Work Environment, National Institute of Occupational Health, Pb 8149 Dep., N-0033 Oslo, Norway.
Department of Industrial Process, Technology SINTEF Materials and Chemistry, PB 4760, N-7465 Trondheim, Norway.
Int J Mol Sci. 2019 Feb 10;20(3):740. doi: 10.3390/ijms20030740.
Tremendous efforts are applied in the ferroalloy industry to control and reduce exposure to dust generated during the production process, as inhalable Mn-containing particulate matter has been linked to neurodegenerative diseases. This study aimed to investigate the toxicity and biological effects of dust particles from laboratory-scale processes where molten silicomanganese (SiMn) was exposed to air, using a human astrocytoma cell line, 1321N1, as model system. Characterization of the dust indicated presence of both nano-sized and larger particles averaging between 100 and 300 nm. The dust consisted mainly of Si, Mn and O. Investigation of cellular mechanisms showed a dose- and time-dependent effect on cell viability, with only minor changes in the expression of proteins involved in apoptosis. Moreover, gene expression of the neurotoxic biomarker () increased, whereas APP protein expression decreased. Finally, induction of gap junctional intercellular communication (GJIC) increased with higher doses and correlated with the other endpoints. Thus, the effects of SiMn dust on 1321N1 cells are highly dependent on the dose of exposure and involves changes in APP, apoptosis-related proteins and intercellular communication.
在铁合金行业，人们付出了巨大努力来控制和减少生产过程中产生的粉尘暴露，因为可吸入的含锰颗粒物与神经退行性疾病有关。本研究旨在利用人星形胶质细胞瘤细胞系 1321N1 作为模型系统，研究在实验室规模下熔融硅锰（SiMn）暴露于空气中时产生的粉尘颗粒的毒性和生物学效应。对粉尘的特性分析表明，其存在纳米级和较大粒径的颗粒，平均粒径在 100 至 300nm 之间。该粉尘主要由 Si、Mn 和 O 组成。细胞机制的研究表明，细胞活力具有剂量和时间依赖性效应，而参与细胞凋亡的蛋白表达仅有轻微变化。此外，神经毒性生物标志物（）的基因表达增加，而 APP 蛋白表达减少。最后，随着剂量的增加，缝隙连接细胞间通讯（GJIC）的诱导增加，并且与其他终点相关。因此，SiMn 粉尘对 1321N1 细胞的影响高度依赖于暴露剂量，并涉及 APP、凋亡相关蛋白和细胞间通讯的变化。
