Salom Cecilia, Álvarez-Teijeiro Saúl, Fernández M Pilar, Morgan Reginald O, Allonca Eva, Vallina Aitana, Lorz Corina, de Villalaín Lucas, Fernández-García M Soledad, Rodrigo Juan P, García-Pedrero Juana M
Department of Otolaryngology, Hospital Universitario Central de Asturias and Instituto de Investigación Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain.
CIBERONC, Av. Monforte de Lemos, 3-5. 28029, Madrid, Spain.
J Clin Med. 2019 Feb 10;8(2):229. doi: 10.3390/jcm8020229.
The annexin protein superfamily has been implicated in multiple physiological and pathological processes, including carcinogenesis. Altered expression of various annexins has frequently been observed and linked to the development and progression of various human malignancies. However, information is lacking on the expression and clinical significance of annexin A9 (ANXA9) and A10 (ANXA10) in head and neck squamous cell carcinomas (HNSCC). ANXA9 and ANXA10 expression was evaluated in a large cohort of 372 surgically treated HPV-negative HNSCC patients and correlated with the clinicopathologic parameters and disease outcomes. Down-regulation of ANXA9 expression was found in 42% of HNSCC tissue samples, compared to normal epithelia. ANXA9 expression in tumors was significantly associated with oropharyngeal location and histological differentiation grade ( < 0.001). In marked contrast, ANXA10 expression was absent in normal epithelium, but variably detected in the cytoplasm of cancer cells. Positive ANXA10 expression was found in 64% of tumors, and was significantly associated with differentiation grade ( < 0.001), being also more frequent in oropharyngeal tumors ( = 0.019). These results reveal that the expression of both ANXA9 and ANXA10 is frequently altered in HNSCC and associated to the tumor differentiation grade, suggesting that they could be implicated in the pathogenesis of these cancers.
膜联蛋白超家族与多种生理和病理过程有关，包括肿瘤发生。经常观察到各种膜联蛋白的表达改变，并与各种人类恶性肿瘤的发生和发展有关。然而，关于膜联蛋白A9（ANXA9）和A10（ANXA10）在头颈部鳞状细胞癌（HNSCC）中的表达及临床意义的信息尚缺乏。在372例接受手术治疗的HPV阴性HNSCC患者的大样本队列中评估了ANXA9和ANXA10的表达，并将其与临床病理参数和疾病转归相关联。与正常上皮相比，42%的HNSCC组织样本中发现ANXA9表达下调。肿瘤中的ANXA9表达与口咽部位和组织学分化程度显著相关（<0.001）。与之形成鲜明对比的是，正常上皮中不存在ANXA10表达，但在癌细胞的细胞质中可检测到不同程度的表达。64%的肿瘤中发现ANXA10表达阳性，且与分化程度显著相关（<0.001），在口咽肿瘤中也更常见（=0.019）。这些结果表明，ANXA9和ANXA10在HNSCC中均经常发生表达改变，并与肿瘤分化程度相关，提示它们可能参与了这些癌症的发病机制。
