Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec Kralove, Czech Republic.
Molecules. 2019 Feb 10;24(3):616. doi: 10.3390/molecules24030616.
Lipases are enzymes responsible for the conversion of triglycerides and other esterified substrates, they are involved in the basic metabolism of a wide number of organisms, from a simple microorganism and to mammals. They also have broad applicability in many fields from which industrial biotechnology, the production of cleaning agents, and pharmacy are the most important. The use of lipases in analytical chemistry where it can serve as a part of biosensors or bioassays is an application of growing interest and has become another important use. This review is focused on the description of lipases chemistry, their current applications and the methods for their assay measurement. Examples of bioassays and biosensors, including their physical and chemical principles, performance for specific substrates, and discussion of their relevance, are given in this work.
脂肪酶是负责将甘油三酯和其他酯化底物转化的酶，它们参与了从简单微生物到哺乳动物等大量生物体的基本代谢。它们在许多领域也具有广泛的应用，其中工业生物技术、清洁剂的生产和制药是最重要的领域。脂肪酶在分析化学中的应用，例如作为生物传感器或生物测定的一部分，是一个越来越受到关注的应用领域，并且已经成为另一个重要的应用。本文重点介绍了脂肪酶的化学性质、它们的当前应用以及它们的测定方法。本文还介绍了生物测定和生物传感器的例子，包括它们的物理和化学原理、对特定底物的性能以及对它们相关性的讨论。
