通过原生质体电融合实现柑橘与其野生近缘种九里香之间的体细胞杂种再生植株

Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion.

作者信息

Guo W W, Deng X X

机构信息

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Fax: +86-27-87396057 e-mail:

出版信息

Plant Cell Rep. 1998 Dec;18(3-4):297-300. doi: 10.1007/s002990050575.

DOI:10.1007/s002990050575

PMID:30744239

Abstract

Protoplasts isolated from `Page' tangelo (Minneola tangelo × clementine) cell suspension cultures were electrically fused with mesophyll protoplasts of orange jessamine [Murraya paniculata (L.) Jack]. Shoots were regenerated after 6 - 10 months of culture, but they were extremely recalcitrant to producing roots in root-induction medium. Complete plantlets were formed via micrografting. Chromosome counting of shoot tips revealed they were tetraploids (2n = 4x = 36). Glutamateoxaloacetate transaminase isozyme and randomly amplified polymorphic DNA analysis confirmed their hybridity. Orange jessamine is immune to citrus huanglongbin, a severe disease of citrus, but sexual incompatibility and limited graft compatibility exist between Citrus and orange jessamine. The cell fusion technique may make it possible to transfer the huanglongbin resistance trait from orange jessamine to Citrus.

摘要

从“佩奇”坦吉洛（明尼奥拉坦吉洛×克莱门氏小柑橘）细胞悬浮培养物中分离出的原生质体与九里香[Murraya paniculata (L.) Jack]的叶肉原生质体进行了电融合。培养6 - 10个月后再生出芽，但它们在生根诱导培养基中极难生根。通过微嫁接形成了完整的植株。对茎尖进行染色体计数表明它们是四倍体（2n = 4x = 36）。谷氨酸草酰乙酸转氨酶同工酶和随机扩增多态性DNA分析证实了它们的杂种性。九里香对柑橘黄龙病（一种严重的柑橘病害）具有免疫力，但柑橘与九里香之间存在有性不亲和性和有限的嫁接亲和性。细胞融合技术可能使将黄龙病抗性性状从九里香转移到柑橘成为可能。