Guo W W, Deng X X
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan.
Sheng Wu Gong Cheng Xue Bao. 2000 Mar;16(2):179-82.
Protoplasts isolated from cell suspension cultures of 'Bonnaza' navel orange (Citrus sinensis L. Osbeck) were electrically fused with mesophyll protoplasts of rough lemon (Citrus jambhiri Lush) and Goutou orange (Citrus aurantium L.) respectively. Plants regenerated from both fusion combinations. Chromosome counting of randomly selected fifty two globular embryoids as well as all the regenerated seventy four plants from Bonnaza navel + rough lemon revealed that twenty six embryoids were tetraploids, and the rest were diploids while 100% regenerated plants were tetraploids. The results inferred that somatic hybrids were more competitive than parental genotypes in the process of plant regeneration. All the regenerated 14 plants from Bonnaza navel + Goutou orange were tetraploids as revealed by chromosome counting. POX isozyme and RAPD analysis verified that the plants from Bonnaza navel + rough lemon were hybrids, and RAPD analysis confirmed the hybridity of those from Bonnaza navel + Goutou orange.
从“邦纳扎”脐橙（Citrus sinensis L. Osbeck）细胞悬浮培养物中分离出的原生质体，分别与粗柠檬（Citrus jambhiri Lush）和枸头橙（Citrus aurantium L.）的叶肉原生质体进行电融合。两种融合组合均再生出了植株。对随机选取的52个球形胚状体以及从邦纳扎脐橙 + 粗柠檬组合中再生出的全部74株植株进行染色体计数，结果显示26个胚状体为四倍体，其余为二倍体，而再生植株100%为四倍体。结果推断，体细胞杂种在植株再生过程中比亲本基因型更具竞争力。染色体计数表明，从邦纳扎脐橙 + 枸头橙组合中再生出的14株植株均为四倍体。POX同工酶和RAPD分析证实，邦纳扎脐橙 + 粗柠檬组合的植株为杂种，RAPD分析也证实了邦纳扎脐橙 + 枸头橙组合植株的杂种性。
