Zhu B Q, Gao W Y, Xu L, Gao Y, Shao Z J
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2019 Feb 6;53(2):153-158. doi: 10.3760/cma.j.issn.0253-9624.2019.02.006.
To investigate the molecular characteristics of serogroup B in China. Total of 485 (100 strains isolated from cerebrospinal fluid or blood samples of encephalomyelitis cases, and 385 strains isolated from nasopharynx of healthy carriers) Meningococcal serogroup B (MenB) strains, isolated from 29 provinces of China between 1968 and 2016, were analyzed by multilocus sequence typing (MLST) and PorA typing methods. Further, the genetic diversity of three MenB vaccine proteins, FHbp, NadA and NHBA, were analyzed. The 485 study strains belonged to 270 sequence types (STs), 107 of which (representing 211 strains) could be grouped into ten clonal complexes (CC). CC4821 has been the predominant lineage in China since 2005 (28.7%, 139). The most common PorA types of MenB strains from invasive meningococcal cases were P1.5-2,2-2 (10.0%, 10), P1.5-1,2-2 (9.0%, 9) and P1.5-1,10-4 (9.0%, 9). Four hundred and twenty one strains had intact gene; variant 1, 2 and 3 accounted for 12.8% (54 strains), 85.0% (358 strains) and 2.2% (9 strains) respevtively. Ten out of 432 strains (2.3%) contained complete gene. All the 172 strains for which the gene was sequenced had intact gene sequence which corresponded to 68 peptide types. CC4821 was the predominant CC of MenB strains in China; the vaccine proteins were diverse about the sequences. The vaccine proteins should be carefully selected when developing MenB vaccines in China.
为研究中国B群脑膜炎奈瑟菌的分子特征。对1968年至2016年间从中国29个省份分离出的485株B群脑膜炎奈瑟菌（MenB）菌株（其中100株从脑脊髓炎病例的脑脊液或血液样本中分离，385株从健康携带者的鼻咽部分离）采用多位点序列分型（MLST）和PorA分型方法进行分析。此外，还分析了三种MenB疫苗蛋白FHbp、NadA和NHBA的遗传多样性。485株研究菌株属于270个序列型（STs），其中107个（代表211株）可归为10个克隆复合体（CC）。自2005年以来，CC4821一直是中国的主要谱系（28.7%，139株）。侵袭性脑膜炎球菌病例中MenB菌株最常见的PorA型为P1.5-2,2-2（10.0%，10株）、P1.5-1,2-2（9.0%，9株）和P1.5-1,10-4（9.0%，9株）。421株具有完整基因；变体1、2和3分别占12.8%（54株）、85.0%（358株）和2.2%（9株）。432株中有10株（2.3%）含有完整基因。对其基因进行测序的172株均具有完整的基因序列，对应68种肽型。CC4821是中国MenB菌株的主要CC；疫苗蛋白的序列具有多样性。在中国研发MenB疫苗时应谨慎选择疫苗蛋白。
