Santos Dércia, Luzio Ana, Coimbra Ana M, Varandas Simone, Fontaínhas-Fernandes António, Monteiro Sandra M
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD),Quinta de Prados, 5000-801 Vila Real,Portugal.
Departamentode Ciências Florestais e Arquitetura Paisagista (CIFAP),Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências Agrárias e Veterinárias (ECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD),Quinta de Prados, 5000-801 Vila Real,Portugal.
Microsc Microanal. 2019 Feb;25(1):236-243. doi: 10.1017/S1431927618015490. Epub 2019 Feb 12.
Gill histopathology is a valuable tool to evaluate ecosystems health, because the gill is a key site of waterborne pollutant uptake and the first target organ to their toxicity. Consequently, this makes it important in biomonitoring programs. This study aims to evaluate gill histopathological differences in Douro basin native fish species and determine possible associations with water quality and the ecological status classifications. Two native fish species (Pseudochondrostoma duriense and Luciobarbus bocagei) were sampled in four points of the Douro basin: two reference points, Ameixiosa (Paiva River) and Covelas (Bestança River), both classified with an excellent ecological status; and two disturbed points, Castro Daire (Paiva River) and Alvações do Corgo (Corgo River), categorized with a good and a moderate ecological status, respectively. Gill histopathological differences were qualitative and quantitatively analyzed. The histological analysis showed that, in all sampling locations, both species presented some degree of gill differences, such as epithelial lifting, lamellar fusion, and/or necrosis. The histopathological differences evaluation emphasized some variances in the responses between the two species. In nase, the filament and lamellar epithelium proliferation were the histopathological differences that better reflected the river ecological status classification, proving their usefulness in biomonitoring programs.
鳃组织病理学是评估生态系统健康的一项重要工具，因为鳃是水体污染物摄入的关键部位，也是污染物毒性作用的首要靶器官。因此，这使其在生物监测项目中具有重要意义。本研究旨在评估杜罗河流域本地鱼类的鳃组织病理学差异，并确定其与水质及生态状况分类之间可能存在的关联。在杜罗河流域的四个地点采集了两种本地鱼类样本：两个参考点，阿梅肖萨（帕伊瓦河）和科韦拉斯（贝斯坦萨河），二者的生态状况均被评为优良；以及两个受干扰点，卡斯特罗代雷（帕伊瓦河）和阿尔瓦绍斯杜尔戈（杜尔戈河），其生态状况分别被归类为良好和中等。对鳃组织病理学差异进行了定性和定量分析。组织学分析表明，在所有采样地点，两种鱼类均呈现出一定程度的鳃差异，如上皮隆起、鳃小片融合和/或坏死。组织病理学差异评估强调了两种鱼类在反应上的一些差异。对于雅罗鱼，鳃丝和鳃小片上皮细胞增殖是最能反映河流生态状况分类的组织病理学差异，证明了它们在生物监测项目中的实用性。
