Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, CZ-166 28 Prague 6, Czech Republic.
Research and Breeding Institute of Pomology Holovousy, Department of Genebanks, Holovousy 129, Horice 508 01, Czech Republic.
Food Chem. 2019 Jun 30;284:162-170. doi: 10.1016/j.foodchem.2019.01.014. Epub 2019 Jan 11.
Due to unique phytochemicals contained, Vaccinum berries are known to have a number of positive health effects. In this context, lingonberries (Vaccinium vitis-idaea) are considered to be the most effective, thus finding many uses. Recently, fraud suspicion on lingonberries-based products has been reported, partial or even total replacement by less valued cranberries (Vaccinium macrocarpon) was found. In this study, metabolomic fingerprinting employing instrumental platform consisting of U-HPLC-HRMS/MS was investigated for discrimination between the two Vaccinum berries species. Methanolic extracts of 33 authentic samples from two harvest years were analyzed and chemometric evaluation was performed to identify significant marker compounds, their stability during drying process was assessed, too. The characteristic markers most contributing to berries classification were representatives of polyphenols and phospholipids. Peonidin 3-O-arabinoside and myricetin 3-O-glucoside, not occurring in lingonberries, enabled to discover the presence of cranberries in prepared admixtures down to 1% (w/w).
由于所含的独特植物化学成分，越橘浆果被认为对健康有许多积极影响。在这方面，蔓越莓（Vaccinium vitis-idaea）被认为是最有效的，因此有许多用途。最近，据报道有人怀疑以蔓越莓为基础的产品存在欺诈行为，发现部分甚至全部用价值较低的酸果蔓（Vaccinium macrocarpon）替代。在这项研究中，采用由 U-HPLC-HRMS/MS 组成的仪器平台进行代谢组指纹图谱分析，以区分两种越橘浆果。对来自两个收获年份的 33 个真实样本的甲醇提取物进行了分析，并进行了化学计量评估，以确定显著的标记化合物，还评估了它们在干燥过程中的稳定性。对浆果分类贡献最大的特征标记物是多酚和磷脂的代表。在蔓越莓中不存在的矢车菊素 3-O-阿拉伯糖苷和杨梅素 3-O-葡萄糖苷，能够发现制备混合物中酸果蔓的存在，低至 1%（w/w）。
