Cellular and Molecular Research Center, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Department of Life Sciences, Faculty of New Sciences and Technologies, University of Tehran, Iran.
Food Chem. 2019 Jun 30;284:334-339. doi: 10.1016/j.foodchem.2019.01.111. Epub 2019 Jan 24.
Protein composition and molecular weight play an important role in the digestibility of microalgae proteins. In this study for the first time, proteinous materials of Dunaliella salina and Spirulina platensis were extracted and purified by fast protein liquid chromatography. Then, they are affected by trypsin and chymotrypsin as indicator intestinal enzymes. The results showed that the extracted protein from S. plantesis (ProS) was more rapidly hydrolysed than proteins from D. salina (ProD) because of their lower molecular weight and likely their greater flexibility and open structure. Also, the extent of hydrolysis by trypsin and chymotrypsin of ProS were higher and faster than ProD due to the more number of hydrolytic sites in ProS for both enzymes. The catalytic efficiency and k displayed that ProS were more suitable substrate than ProD for intestinal enzymes. The results exhibited that chymotrypsin can act better and faster than trypsin on peptide bonds of proteins.
蛋白质组成和分子量在微藻蛋白的消化率中起着重要作用。在这项研究中，首次通过快速蛋白质液相色谱法从盐藻和螺旋藻中提取和纯化了蛋白质。然后，用胰蛋白酶和糜蛋白酶作为指示性肠酶对其进行了影响。结果表明，由于分子量较低，螺旋藻（ProS）中的提取蛋白可能更具灵活性和开放结构，因此比盐藻（ProD）中的蛋白更易被水解。此外，由于 ProS 中两种酶的水解位点更多，因此胰蛋白酶和糜蛋白酶对 ProS 的水解程度更高、速度更快。催化效率和 k 表明，ProS 比 ProD 更适合作为肠酶的底物。结果表明，糜蛋白酶对蛋白质肽键的作用优于胰蛋白酶。
