Key Laboratory of Economic and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Pestic Biochem Physiol. 2019 Jan;153:47-54. doi: 10.1016/j.pestbp.2018.11.001. Epub 2018 Nov 3.
Lambda-cyhalothrin has long been recommended as an effective insecticide to control the oriental armyworm, Mythimna separata (Walker), a notorious migratory pest of agricultural plants. Previous researches have suggested that survival, development, and reproduction of insects are influenced by sublethal concentrations of insecticides. However, the effects of sublethal concentrations of lambda-cyhalothrin on M. separata are less known. In this study, we determined the toxicity and effects of LC and LC concentration of lambda-cyhalothrin on development and reproduction of M. separata. Results indicate that LC of lambda-cyhalothrin tends to decrease the life traits of M. separate, with a shortening larvae period of offspring and oviposition period, whereas LC of lambda-cyhalothrin stimulates daily maximal fecundity and forwards the oviposition peak, suggesting a stimulation of reproduction by LC of lambda-cyhalothrin. The M. separata population was increased by an LC concentration of lambda-cyhalothrin, resulting in a net reproductive rate (R) and intrinsic rate of increase (r) significantly higher than that of the control. Transcripts of vitellogenin (MsVg) and vitellogenin receptor (MsVgR) genes were suppressed at day 1 after emergence of moth which developed from the larvae exposed to LC and LC of lambda-cyhalothrin, but were significant induced when the moth begin to lay eggs (day 4), with a more remarkable induction by LC of lambda-cyhalothrin than those of LC of lambda-cyhalothrin. Our results indicate that the observed stimulation of reproduction is therefore the results of up-regulation of MsVg and MsVgR by LC of lambda-cyhalothrin.
氯氟氰菊酯长期以来被推荐作为一种有效的杀虫剂，用于防治东方粘虫，Mythimna separata（Walker），一种对农业植物具有严重迁徙性的害虫。先前的研究表明，昆虫的生存、发育和繁殖受到亚致死浓度杀虫剂的影响。然而，亚致死浓度的氯氟氰菊酯对 M. separata 的影响知之甚少。在本研究中，我们测定了 LC 和 LC 浓度的氯氟氰菊酯对 M. separata 发育和繁殖的毒性和影响。结果表明，LC 浓度的氯氟氰菊酯倾向于降低 M. separate 的生命特征，使后代幼虫期和产卵期缩短，而 LC 浓度的氯氟氰菊酯刺激每日最大产卵量，并提前产卵高峰，表明 LC 浓度的氯氟氰菊酯刺激繁殖。LC 浓度的氯氟氰菊酯增加了 M. separata 种群，导致净生殖率（R）和内禀增长率（r）显著高于对照。暴露于 LC 和 LC 浓度的氯氟氰菊酯的幼虫发育成的蛾在出茧后第 1 天，其卵黄蛋白原（MsVg）和卵黄蛋白原受体（MsVgR）基因的转录物被抑制，但当蛾开始产卵（第 4 天）时，转录物显著诱导，LC 浓度的氯氟氰菊酯比 LC 浓度的氯氟氰菊酯诱导更为明显。我们的结果表明，观察到的繁殖刺激是由于 LC 浓度的氯氟氰菊酯对 MsVg 和 MsVgR 的上调所致。
