Özbek Mehmet, Bayraktaroğlu Alev Gürol
Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Burdur, Turkey.
Ankara University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Ankara, Turkey.
Acta Histochem. 2019 Apr;121(3):311-322. doi: 10.1016/j.acthis.2019.01.005. Epub 2019 Feb 10.
Peyer's patches are known as the immune sensors of the intestine because of their ability to transport luminal antigens. The objective of this study was both to assess the prenatal and postnatal development of sheep ileal Peyer's patches with respect to histomorphology, distribution of CD4 and CD8 cells, and localization of proliferating and apoptotic cells, and to examine the morphology of M cells and expression of CK18 in follicle associated epithelium (FAE). We also hypothesized that CK18 could be a potential marker for M cell. Peyer's patches completed their histomorphological development in prenatal period and involuted in the postnatal period. The distribution of the CD4 and CD8 cells was similar in the last trimester of pregnancy (days 120-150) and the postnatal period, but differed in the early stages of foetal development (days 70-120). In the prenatal period, the follicular area displayed high levels of proliferation and apoptosis. We observed CK18 immunoreaction only in FAE. While M cells were devoid of microfolds in the early stages of the prenatal period, these cells acquired a prismatic shape and bore distinct apical microfolds in the late prenatal period and postnatal period. As a result, it was determined that, in sheep, the development of the ileal Peyer's patches occurred in the prenatal period, independent of exogenous antigenic stimulation, and in association with high levels of lymphopoiesis and apoptosis in the follicles. We found, for the first time, that CK18 is a novel and reliable marker for FAE in sheep ileal Peyer's patches. We suggest that CK18 positive cells in FAE are M cells.
派尔集合淋巴结因其运输肠腔抗原的能力而被称为肠道的免疫传感器。本研究的目的是评估绵羊回肠派尔集合淋巴结在组织形态学、CD4和CD8细胞分布、增殖细胞和凋亡细胞定位方面的产前和产后发育情况，并检查M细胞的形态以及卵泡相关上皮（FAE）中细胞角蛋白18（CK18）的表达。我们还假设CK18可能是M细胞的潜在标志物。派尔集合淋巴结在产前完成其组织形态学发育，并在产后退化。妊娠晚期（120 - 150天）和产后CD4和CD8细胞的分布相似，但在胎儿发育早期（70 - 120天）有所不同。在产前阶段，滤泡区域显示出高水平的增殖和凋亡。我们仅在FAE中观察到CK18免疫反应。虽然在产前早期M细胞没有微褶，但在产前后期和产后这些细胞呈现棱柱形并带有明显的顶端微褶。结果表明，在绵羊中，回肠派尔集合淋巴结的发育发生在产前阶段，独立于外源性抗原刺激，并且与滤泡中高水平的淋巴细胞生成和凋亡有关。我们首次发现CK18是绵羊回肠派尔集合淋巴结中FAE的一种新型且可靠的标志物。我们认为FAE中CK18阳性细胞是M细胞。
