Association of Growth Factors Genes with Miscarriage.

BACKGROUND

The study was aimed to investigate the association of V gene polymorphic variants - (rs699947) and - (rs2010963) and gene (rs1800471) and gene expression level with miscarriage in the first trimester.

METHODS

288 women with different courses of pregnancy and 61 chorionic tissue samples were involved in case-control study. Allele-specific polymerase chain reaction in real time was used for genotyping. Next, gene-gene interactions were analyzed using the multifactor dimensionality reduction method. and genes expression levels were determined by RT-PCR.

RESULTS

It was found that SNP rs699947 was associated with the miscarriage risk change (p=0.05). The genotype was associated with reduced risk of abortion in the first trimester, and the genotype with increased risk. Genotypes and were associated with lowered risk of miscarriage in the first trimester. mRNA level of was significantly higher in decidual tissue compared to chorionic tissue in normally progressing pregnancy (p=0.003). gene expression level was directly correlated with the TGFB1 mRNA level (R=0.60; p=0.038). In pregnancy loss, an inverse relationship was observed (R=-0.76; p=0.028).

CONCLUSION

The SNP rs699947 is associated with pregnancy loss in the first trimester. The MDR analysis data showed the significant relationship between and genes in two-locus and three-locus models. A change in the ratio of the concentrations of growth factors can disrupt the processes of cell division, apoptosis and angiogenesis processes.