Gkagkavouzis Konstantinos, Karaiskou Nikoleta, Katopodi Theodora, Leonardos Ioannis, Abatzopoulos Theodore J, Triantafyllidis Alexander
Department of Genetics, Development & Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Department of Biological Sciences and Preventive Medicine, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Fish Biol. 2019 Apr;94(4):606-613. doi: 10.1111/jfb.13932. Epub 2019 Mar 1.
We examined 662 gilthead sea bream Sparus aurata from wild samples of the species in the Aegean and Ionian Seas, using 20 EST-linked microsatellite markers, in three multiplex panels, as well as seven anonymous loci. Most of the markers were revealed to be highly polymorphic. We found low genetic differentiation between the sampling stations/areas with total F 0.002 (P < 0.05). Based on comparison of five temporal samples, our results indicate genetic data consistency over time for all tested samples, pointing to stable populations, despite reported repeated escape events. Our results confirm the genetic population structure previously observed in these specific areas, using by far more markers than in previous studies in both coding and non-coding DNA loci. The limited genetic structure and the temporal genetic stability indicate neither major genetic differentiation of local populations by geographic isolation nor influence from anthropogenic factors. These results provide a baseline for future reference in any management programme of both wild and farmed population of S. aurata as well as of other aquaculture species with a potential introgression among farmed and wild populations.
我们使用20个EST连锁微卫星标记（分三个多重面板）以及7个匿名位点，对来自爱琴海和爱奥尼亚海该物种野生样本的662条金头鲷进行了检测。结果显示，大多数标记具有高度多态性。我们发现采样站/区域之间的遗传分化较低，总F值为0.002（P < 0.05）。基于对五个时间样本的比较，我们的结果表明，所有测试样本的遗传数据随时间保持一致，尽管有报道称存在多次逃逸事件，但这表明种群稳定。我们的结果证实了先前在这些特定区域观察到的遗传种群结构，在编码和非编码DNA位点上使用的标记数量远远超过以往研究。有限的遗传结构和时间上的遗传稳定性表明，当地种群既没有因地理隔离而产生重大遗传分化，也没有受到人为因素的影响。这些结果为未来金头鲷野生和养殖种群以及其他养殖和野生种群可能存在基因渗入的水产养殖物种的任何管理计划提供了基线参考。
