Kitasato University School of Marine Biosciences, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa, 252-0373, Japan.
Department of Regional Agricultural Engineering, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Nakagusuku, Okinawa, 903-0213, Japan.
Mar Biotechnol (NY). 2019 Apr;21(2):291-300. doi: 10.1007/s10126-019-09880-3. Epub 2019 Feb 12.
Coral reef degradation due to various local stresses, such as nutrient enrichment and terrestrial run-off into coastal waters, is an increasing global concern. Inorganic phosphates have been considered to possibly inhibit skeleton formation in corals. Despite many studies available on the effects of nutrients on corals, a clear consensus on how nutrients exert deteriorative effects on corals has not been established satisfactorily. In this study, we examined the effects of phosphates and nitrates on in vitro aragonite CaCO formation by using biogenic polyamines and in vivo aragonite formation in the skeleton of juvenile Acropora digitifera corals. We showed that the phosphates at similar concentrations clearly inhibited both in vitro and in vivo CaCO formation. In contrast, nitrates inhibited neither in vitro aragonite CaCO formation nor in vivo aragonite formation in juvenile coral skeleton. Furthermore, our findings showed that inhibition of coral skeleton formation was due to absorption of phosphate on the skeleton, which inorganically inhibited normal development of juvenile coral skeleton.
由于各种局部压力，如营养物质富化和陆地径流进入沿海水域，珊瑚礁退化是一个日益严重的全球性问题。无机磷酸盐被认为可能会抑制珊瑚的骨骼形成。尽管有许多关于营养物质对珊瑚影响的研究，但关于营养物质如何对珊瑚产生破坏性影响的问题尚未得到令人满意的明确共识。在这项研究中，我们使用生物源多胺研究了磷酸盐和硝酸盐对体外方解石 CaCO3 形成的影响，并研究了在有活体幼年鹿角珊瑚骨骼中方解石形成的影响。我们发现，相似浓度的磷酸盐明显抑制了体外和体内 CaCO3 的形成。相比之下，硝酸盐既不抑制体外方解石 CaCO3 的形成，也不抑制活体幼年珊瑚骨骼中方解石的形成。此外，我们的研究结果表明，珊瑚骨骼形成的抑制是由于磷酸盐在骨骼上的吸收，这抑制了幼年珊瑚骨骼的正常发育。
